EST-derived single nucleotide polymorphism markers for assembling genetic and physical map of the barley genome

Abstract

In a panel of seven genotypes, 437 expressed sequence tag (EST)-derived DNA fragments were sequenced. Single nucleotide polymorphisms (SNPs) that were polymorphic between the parents of three mapping populations were mapped by heteroduplex analysis and a genome-wide consensus map comprising 216 EST-derived SNPs and 4 InDel (insertion/deletion) markers was constructed. The average frequency of SNPs amounted to 1/130 bp and 1/107.8 bp for a set of randomly selected and a set of mapped ESTs, respectively.

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Keywords

Molecular markers, SNPs, Haplotype diversity, Nucleotide diversity, Genetic map

Citation

Funct. Integr. Genomics, 8: 223-233

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