Browsing by Author "Varshney, R. K."

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    EST-derived single nucleotide polymorphism markers for assembling genetic and physical map of the barley genome
    (Springer, 2008) Kota, R.; Varshney, R. K.; Prasad, M.; Zhang, H.; Stein, N.; Graner, A.
    In a panel of seven genotypes, 437 expressed sequence tag (EST)-derived DNA fragments were sequenced. Single nucleotide polymorphisms (SNPs) that were polymorphic between the parents of three mapping populations were mapped by heteroduplex analysis and a genome-wide consensus map comprising 216 EST-derived SNPs and 4 InDel (insertion/deletion) markers was constructed. The average frequency of SNPs amounted to 1/130 bp and 1/107.8 bp for a set of randomly selected and a set of mapped ESTs, respectively.