Improved method of RNA isolation from laser capture microdissection (LCM)-derived plant tissues

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dc.contributor.authorGautam, Vibhav
dc.contributor.authorSingh, Archita
dc.contributor.authorSingh, Sharmila
dc.contributor.authorVerma, Swati
dc.contributor.authorSarkar, Ananda K.
dc.date.accessioned2019-04-11T10:22:19Z
dc.date.available2019-04-11T10:22:19Z
dc.date.issued2019
dc.descriptionAccepted date: 04 April 2019en_US
dc.description.abstractLaser capture microdissection (LCM) is a tool to isolate desired and/or less accessible cells or tissues from a heterogeneous population. In the current method, we describe an efficient and cost-effective method to obtain both high-quality mRNA and miRNAs in sufficient quantity from LCM-derived plant tissues. The quality of the isolated RNA can be assessed using Bioanalyzer. Using modified stem-loop RT-PCR, we confirmed the presence of 21–24 nucleotide (nt) long mature miRNAs. This modified LCM-based method has been found to be suitable for the tissue-specific expression analysis of both genes and small RNAs (miRNAs).en_US
dc.description.sponsorshipV.G., A.S., and S.S. thank the Council for Scientific and Industrial Research (CSIR), India, and the National Institute of Plant Genome Research (NIPGR), New Delhi, India, for funding and internal grants. V.G. and A.S. also acknowledge the Department of Biotechnology (DBT), India, for fellowship. S.V. thanks the Department of Science and Technology-Science and Engineering Research Board (DST-SERB), India, for National Post Doctoral Fellowship (N-PDF). A.K.S. thanks NIPGR and DBT (Project Grant No. BT/PR12766/BPA/118/63/2015), New Delhi, India, for fellowship and grants. The authors declare no conflict of interests.en_US
dc.identifier.citationMethods in Molecular Biology, 1933: 89-98en_US
dc.identifier.doi10.1007/978-1-4939-9045-0_5.en_US
dc.identifier.isbn978-1-4939-9045-0
dc.identifier.officialurlhttps://link.springer.com/protocol/10.1007%2F978-1-4939-9045-0_5en_US
dc.identifier.urihttps://ndkr-library.nipgr.ac.in/handle/123456789/940
dc.language.isoen_USen_US
dc.publisherSpringer Natureen_US
dc.subjectLCMen_US
dc.subjectLaser microdissectionen_US
dc.subjectRNA isolationen_US
dc.subjectGene expressionen_US
dc.subjectmiRNAen_US
dc.subjectRoot meristemen_US
dc.titleImproved method of RNA isolation from laser capture microdissection (LCM)-derived plant tissuesen_US
dc.typeBook chapteren_US

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