MicroRNA397 regulates tolerance to drought and fungal infection by regulating lignin deposition in chickpea root

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dc.contributor.authorSharma, Nilesh Kumar
dc.contributor.authorYadav, Shalini
dc.contributor.authorGupta, Santosh Kumar
dc.contributor.authorIrulappan, Vadivelmurugan
dc.contributor.authorFrancis, Aleena
dc.contributor.authorSenthil-Kumar, Muthappa
dc.contributor.authorChattopadhyay, Debasis
dc.date.accessioned2023-07-11T09:11:08Z
dc.date.available2023-07-11T09:11:08Z
dc.date.issued2023
dc.descriptionAccepted date: 28 June 2023en_US
dc.description.abstractPlants deposit lignin in the secondary cell wall as a common response to drought and pathogen attacks. Cell wall localised multicopper oxidase family enzymes LACCASES (LACs) catalyse the formation of monolignol radicals and facilitate lignin formation. We show an upregulation of the expression of several LAC genes and a downregulation of microRNA397 (CamiR397) in response to natural drought in chickpea roots. CamiR397 was found to target LAC4 and LAC17L out of twenty annotated LACs in chickpea. CamiR397 and its target genes are expressed in the root. Overexpression of CamiR397 reduced expression of LAC4 and LAC17L and lignin deposition in chickpea root xylem causing reduction in xylem wall thickness. Downregulation of CamiR397 activity by expressing a short tandem target mimic (STTM397) construct increased root lignin deposition in chickpea. CamiR397-overexpressing and STTM397 chickpea lines showed sensitivity and tolerance, respectively, towards natural drought. Infection with a fungal pathogen Macrophomina phaseolina, responsible for dry root rot (DRR) disease in chickpea, induced local lignin deposition and LAC gene expression. CamiR397-overexpressing and STTM397 chickpea lines showed more sensitivity and tolerance, respectively, to DRR. Our results demonstrated the regulatory role of CamiR397 in root lignification during drought and DRR in an agriculturally important crop chickpea.en_US
dc.description.sponsorshipThe assistance of central instrumentation facilities, metabolomics facility and DISC of NIPGR in various experiments is acknowledged. The authors are grateful to the DBT‐eLibrary Consortium (DeLCON) for providing access to e‐Resources. The project is funded by the National Institute of Plant Genome Research, Department of Biotechnology (DBT), Ministry of Science and Technology, Government of India (BT/PR3304/AGR/02/ 815/2011). DC acknowledges J.C. Bose Fellowship (JCB/2020/ 000014) from the Science and Engineering Research Board, Department of Science and Technology. NKS and VI acknowledge DBT, SY and AF acknowledge fellowships from the Council of Scientific and Industrial Research (CSIR, Government of India). The authors are grateful to Dr. Kaisa Kajala, Utrecht University, Netherlands for scientific discussions.en_US
dc.identifier.citationPlant, Cell & Environment, 46(11): 3501-3517en_US
dc.identifier.issn0140-7791
dc.identifier.issn1365-3040
dc.identifier.otherhttps://doi.org/10.1111/pce.14666
dc.identifier.urihttps://onlinelibrary.wiley.com/doi/10.1111/pce.14666
dc.identifier.urihttps://ndkr-library.nipgr.ac.in/handle/123456789/1496
dc.language.isoen_USen_US
dc.publisherJohn Wiley & Sonsen_US
dc.subjectCamiR397en_US
dc.subjectCicer arietinum Len_US
dc.subjectdroughten_US
dc.subjectdry root roten_US
dc.subjectlaccaseen_US
dc.subjectligninen_US
dc.subjectMacrophomina phaseolinaen_US
dc.subjectSTTMen_US
dc.titleMicroRNA397 regulates tolerance to drought and fungal infection by regulating lignin deposition in chickpea rooten_US
dc.typeArticleen_US

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