Level of endogenous free amino acids during various stages of culture of Vigna mungo (L.) Hepper - somatic embryogenesis, organogenesis and plant regeneration

Abstract

A comparative study on endogenous free amino acid levels was conducted in embryogenic and regenerating tissues vis-à-vis regenerated shoots of Vigna mungo PS1. A number of important amino acids were found to increase during initiation of embryogenesis and regeneration. To study the effects of exogenous application of amino acids in this system, media were supplemented with three amino acids, tryptophan (Trp), serine (Ser) and proline (Pro). We found that in the presence of proline, a stress associated amino acid, there was an enhancement in shoot-bud regeneration as well as in the production of somatic embryos. We also analysed the endogenous levels of amino acids in these tissues. This communication thus deals with the induction of globular embryo by auxin and its promotion by proline in V. mungo.