Isolation and characterization of sequence-tagged microsatellite sites markers in chickpea (Cicer arietinum L.)
| dc.contributor.author | Sethy, Niroj K. | |
| dc.contributor.author | Shokeen, Bhumika | |
| dc.contributor.author | Bhatia, Sabhyata | |
| dc.date.AcceptedDate | 6 May 2003 | en_US |
| dc.date.accessioned | 2013-10-22T06:44:46Z | |
| dc.date.available | 2013-10-22T06:44:46Z | |
| dc.date.issued | 2003 | |
| dc.description.abstract | In this study we report the isolation of microsatellite sequences and their conversion to sequence-tagged microsatellite sites (STMS) markers in chickpea (Cicer arietinum L.). Thirteen putative recombinants isolated from a chickpea genomic library were sequenced, and used to design 10 STMS primer pairs. These were utilized to analyse the genetic polymorphism in 15 C. arietinum varieties and two wild varieties, C. echinospermum and C. reticulatum. All the primer pairs amplified polymorphic loci ranging from four to seven alleles per locus. The observed heterozygosity ranged from 0 to 0.6667. Most of the STMS markers also amplified corresponding loci in the wild relatives suggesting conservation of these markers in the genus. Hence, these polymorphic markers will be useful for the evaluation of genetic diversity and molecular mapping in chickpea. | en_US |
| dc.description.sponsorship | The authors thankfully acknowledge NCPGR, an autonomous institute of the Department of Biotechnology, Government of India, for supporting this work. | en_US |
| dc.identifier.citation | Mol. Ecology Notes, 3: 428-430 | en_US |
| dc.identifier.uri | http://hdl.handle.net/123456789/34 | |
| dc.language.iso | en | en_US |
| dc.publisher | Wiley-Blackwell | en_US |
| dc.subject | Chickpea | en_US |
| dc.subject | Cicer arietinum | en_US |
| dc.subject | microsatellites | en_US |
| dc.subject | sequence-tagged microsatellite sites | en_US |
| dc.subject | polymorphism | en_US |
| dc.subject | cross-species amplification | en_US |
| dc.title | Isolation and characterization of sequence-tagged microsatellite sites markers in chickpea (Cicer arietinum L.) | en_US |
| dc.type | Article | en_US |