Publications of NIPGR Scientists

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Subject: search.filters.subject.Proteomic landscape

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    Dehydration-induced proteomic landscape of mitochondria in chickpea reveals large-scale coordination of key biological processes
    (Elsevier B.V, 2019) Gayen, Dipak; Gayali, Saurabh; Barua, Pragya; Lande, Nilesh Vikram; Varshney, Swati; Sengupta, Shantanu; Chakraborty, Subhra; Chakraborty, Niranjan
    Mitochondria play crucial roles in regulating multiple biological processes particularly electron transfer and energy metabolism in eukaryotic cells. Exposure to water-deficit or dehydration may affect mitochondrial function, and dehydration response may dictate cell fate decisions. iTRAQ-based quantitative proteome of a winter legume, chickpea, demonstrated the central metabolic alterations in mitochondria, presumably involved in dehydration adaptation. Three-week-old chickpea seedlings were subjected to progressive dehydration and the magnitude of dehydration-induced compensatory physiological responses was monitored in terms of physicochemical characteristics and mitochondrial architecture. The proteomics analysis led to the identification of 40 dehydration-responsive proteins whose expressions were significantly modulated by dehydration. The differentially expressed proteins were implicated in different metabolic processes, with obvious functional tendencies toward purine-thiamine metabolic network, pathways of carbon fixation and oxidative phosphorylation. The linearity of dehydration-induced proteome alteration was examined with transcript abundance of randomly selected candidates under multivariate stress conditions. The differentially regulated proteins were validated through sequence analysis. An extensive sequence based localization prediction revealed >62.5% proteins to be mitochondrial resident by, at least, one prediction algorithm. The results altogether provide intriguing insights into the dehydration-responsive metabolic pathways and useful clues to identify crucial proteins linked to stress tolerance. BIOLOGICAL SIGNIFICANCE: Investigation on plant mitochondrial proteome is of significance because it would allow a better understanding of mitochondrial function in plant adaptation to stress. Mitochondria are the unique organelles, which play a crucial role in energy metabolism and cellular homeostasis, particularly when exposed to stress conditions. Chickpea is one of the cultivated winter legumes, which enriches soil nitrogen and has very low water footprint and thus contributes to fortification of sustainable agriculture. We therefore examined the dehydration-responsive mitochondrial proteome landscape of chickpea and queried whether molecular interplay of mitochondrial proteins modulate dehydration tolerance. A total of 40 dehydration-induced mitochondrial proteins were identified, predicted to be involved in key metabolic processes. Our future efforts would focus on understanding both posttranslational modification and processing for comprehensive characterization of mitochondrial protein function. This approach will facilitate mining of more biomarkers linked to the tolerance trait and contribute to crop adaptation to climate change.
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    Variety-specific nutrient acquisition and dehydration-induced proteomic landscape of grasspea (Lathyrus sativus L.)
    (Elsevier B.V., 2018) Rathi, Divya; Pareek, Akanksha; Gayali, Saurabh; Chakraborty, Subhra; Chakraborty, Niranjan
    Grasspea, a stress-resilient pulse crop, has largely remained outside the realm of phytochemical and functional genomics analyses despite its high nutritional significance. To unravel the intervarietal variability in nutrient acquisition of grasspea, we conducted a series of physicochemical experiments using two cultivated varieties, LP-24 and Prateek. The analyses revealed high percentage of starch, cellulose, peroxides, carotenoids, phytic acid and minerals in cv. LP-24, whereas large amounts of protein, soluble carbohydrates and antioxidants in Prateek. To dissect the mechanism of stress tolerance, 3-week-old seedlings of cv. LP-24 and Prateek were afflicted with dehydration for a period of 144 h. The physicochemical indices indicated better adaptation in cv. LP-24, with high abundance of proline, phenolics and flavonoids. Dehydration-responsive proteome landscape of cv. LP-24 revealed 152 proteins with variance at a statistically 94% significance level. The comparative proteomics analysis led to the identification of 120 dehydration-responsive proteins (DRPs), most of which were associated with carbohydrate metabolism, amino acid synthesis, antioxidant reactions and cell defense. We report, for the first time, the dehydration-induced proteome landscape of grasspea, whose genome is yet to be sequenced. The results provide unique insights into variety-specific nutrient acquisition attributes and dehydration-tolerance of grasspea. BIOLOGICAL SIGNIFICANCE: Grasspea is a great source of protein and antioxidants with nitrogen fixing ability, besides its tolerance to multivariate environmental stress as compared to major legume species. This represents the first report on nutrient profile and health-promoting attributes of grasspea. The cultivars under study are nutritionally enriched that possess high protein, amino acids and health-promoting factors and may therefore be projected as a vital part of a healthy diet. Grasspea is known for its hardy nature, water-use efficiency and efficacy as a stress-tolerant pulse. Further, this study portrays the dehydration-responsive proteomic landscape of grasspea. The proteomics analyses provide crucial insights into the dehydration response, presumably orchestrated by proteins belonging to an array of functional classes including photosynthesis, protein and RNA metabolism, protein folding, antioxidant enzymes and defense. The interplay of the differentially regulated proteins might aid in reinforcing the mechanisms of dehydration avoidance and/or tolerance.