Publications of NIPGR Scientists

Permanent URI for this communityhttps://ndkr-library.nipgr.ac.in/handle/123456789/1

Browse

Subject: search.filters.subject.Piriformospora indica

Search Results

Now showing 1 - 6 of 6
  • Thumbnail Image
    Item
    Molecular mechanisms of Piriformospora indica mediated growth promotion in plants
    (Taylor & Francis Group, 2022) Kundu, Anish; Vadassery, Jyothilakshmi
    Piriformospora indica is a root endophyte having a vast host range in plants. Plant growth promotion is a hallmark of the symbiotic interaction of P. indica with its hosts. As a plant growth-promoting microorganism, it is important to know the mechanisms involved in growth induction. Hitherto, multiple reports have demonstrated various molecular mechanisms of P. indica-mediated growth promotion, including protein kinase-mediated pathway, enhanced nutrient uptake and polyamine-mediated growth phytohormone elevation. Here, we briefly present a discussion on the state-of-the-art molecular mechanisms of P. indica-mediated growth promotion in host plants, in order to obtain a future prospect on utilization of this microorganism for sustainable agriculture.
  • Thumbnail Image
    Item
    Piriformospora indica recruits host-derived putrescine for growth promotion in plants
    (Oxford University Press, 2022) Kundu, Anish; Mishra, Shruti; Kundu, Pritha; Jogawat, Abhimanyu; Vadassery, Jyothilakshmi
    Growth promotion induced by the endosymbiont Piriformospora indica has been observed in various plants; however, except growth phytohormones, specific functional metabolites involved in P. indica-mediated growth promotion are unknown. Here, we used a GC-MS based untargeted metabolite analysis to identify tomato (Solanum lycopersicum) metabolites whose levels were altered during P. indica-mediated growth promotion. Metabolomic multivariate analysis revealed several primary metabolites with altered levels, with putrescine induced most significantly in roots during the interaction. Further, our results indicated that P. indica modulates the arginine decarboxylase (ADC)-mediated putrescine biosynthesis pathway via induction of SlADC1 in tomato. P. indica did not promote growth in Sladc1-VIGS (virus-induced gene silencing of SlADC1) lines of tomato tomato and showed less colonization. Furthermore, using LC-MS/MS we showed that putrescine promoted growth by elevation of auxin (indole-3-acetic acid) and gibberellin (GA4, GA7) levels in tomato. In Arabidopsis (Arabidopsis thaliana) adc knock-out mutants, P. indica colonization also decreased and showed no plant growth promotion, and this response was rescued upon exogenous application of putrescine. Putrescine is also important for hyphal growth of P. indica, indicating that it is co-adapted by both host and microbe. Taken together, we conclude that putrescine is an essential metabolite and its biosynthesis in plants is crucial for P. indica-mediated plant growth promotion and fungal growth.
  • Thumbnail Image
    Item
    Calcium channel CNGC19 mediates basal defense signaling to regulate colonization of Piriformospora indica in Arabidopsis roots
    (Oxford University Press, 2020) Jogawat, Abhimanyu; Meena, Mukesh Kumar; Kundu, Anish; Varma, Mahendra; Vadassery, Jyothilakshmi
    The activation of calcium signaling is a crucial event for perceiving environmental stress. Colonization by Piriformospora indica, a growth promoting root endosymbiont activates cytosolic Ca2+ in Arabidopsis roots. In this study, we analyze the role of calcium channels responsible for Ca2+ fluxes and its functional relevance. Expression profiling revealed that CNGC19 is a early activated gene, induced by unidentified components in P. indica cell wall extract. Functional analysis revealed that loss-of-function of CNGC19 results in growth inhibition by P.indica, due to increased colonization and loss of controlled P. indica growth. P. indica cell wall extract induced cytosolic Ca2+ elevation is reduced in cngc19 mutant indicating a role in generation of Ca2+cyt elevation. MAMP-trigerred immunity (MTI) is compromised in cngc19 lines as evident from unaltered callose deposition, reduced cis-OPDA, JA and JA-Ile levels and downregulation of jasmonate and other defense related genes which contributes to shift towards pathogenic response. Loss-of-function of CNGC19 results in inability to modulate indole glucosinolate content during P. indica-colonization. CNGC19 mediated basal immunity is AtPep receptor, PEPR dependent. CNGC19 is also crucial for P. indica mediated suppression of AtPep induced immunity. Thus, Arabidopsis CNGC19 is an important Ca2+ channel, maintaining a robust innate immunity and crucial for growth promotion signalling upon P. indica colonization.
  • Thumbnail Image
    Item
    Lectin protein kinase is induced in plant roots in response to the endophytic fungus, Piriformospora indica
    (Springer, 2017) Nivedita; Verma, Praveen K.; Upadhyaya, Kailash C.
    The symbiotic association of Piriformospora indica provides growth promotion leading to increased biomass as well as enhanced plant tolerance to biotic and abiotic stresses in a variety of plant species. Since various protein kinases are thought to be involved in mutual signaling in symbiotic interactions, we analyzed transcript profile of rice protein kinase genes during the infection process by real-time PCR. A rice L-type lectin protein kinase (OslecRK) and Arabidopsis AtLecRK genes are highly upregulated in roots during the initial stage of symbiotic association. In order to investigate the role of LecRK gene in root colonization, the Arabidopsis mutant line (Atlecrk) of rice homolog was used which shows the restricted intracellular penetration of the P. indica hyphae. The results indicate that LecRK might be a crucial factor in perception and recognition during plant–P. indica association by regulating fungal invasion.
  • Thumbnail Image
    Item
    PiHOG1, a stress regulator MAP kinase from the root endophyte fungus Piriformospora indica, confers salinity stress tolerance in rice plants
    (Nature Publishing Group, 2016) Jogawat, Abhimanyu; Vadassery, Jyothilakshmi; Verma, Nidhi; Oelmüller, Ralf; Dua, Meenakshi; Nevo, Eviatar; Johri, Atul Kumar
    In this study, yeast HOG1 homologue from the root endophyte Piriformospora indica (PiHOG1) was isolated and functionally characterized. Functional expression of PiHOG1 in S. cerevisiae ∆hog1 mutant restored osmotolerance under high osmotic stress. Knockdown (KD) transformants of PiHOG1 generated by RNA interference in P. indica showed that genes for the HOG pathway, osmoresponse and salinity tolerance were less stimulated in KD-PiHOG1 compared to the wild-type under salinity stress. Furthermore, KD lines are impaired in the colonization of rice roots under salinity stress of 200 mM NaCl, and the biomass of the host plants, their shoot and root lengths, root number, photosynthetic pigment and proline contents were reduced as compared to rice plants colonized by WT P. indica. Therefore, PiHOG1 is critical for root colonisation, salinity tolerance and the performance of the host plant under salinity stress. Moreover, downregulation of PiHOG1 resulted not only in reduced and delayed phosphorylation of the remaining PiHOG1 protein in colonized salinity-stressed rice roots, but also in the downregulation of the upstream MAP kinase genes PiPBS2 and PiSSK2 involved in salinity tolerance signalling in the fungus. Our data demonstrate that PiHOG1 is not only involved in the salinity response of P. indica, but also helping host plant to overcome salinity stress.
  • Thumbnail Image
    Item
    Piriformospora indica: a friend in need is a friend in deed
    (Research and Reviews, 2016) Trivedi, Dipesh Kumar; Srivastava, Amit; Verma, Praveen K.; Tuteja, Narendra; Gill, Sarvajeet Singh
    Piriformospora indica is an endophytic root-colonizing fungal species classified in the genus Piriformospora of the order Sebacinales. The chlamydospores of P. indica has a typical pear-shape and it was first discovered from the orchid plants in the Thar desert in Rajasthan, India and thus named P. indica.