Publications of NIPGR Scientists

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Subject: search.filters.subject.Hairy roots

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    TAIL-PCR for the recovery of Tnt1 flanking sequences in chickpea: a tool for functional genomics studies
    (Springer Nature Publishing AG, 2026) Chauhan, Chetan; Ranjan, Shubhashish; Jangid, Vinod Kumar; Sinharoy, Senjuti; Senthil-Kumar, Muthappa
    Thermal asymmetric interlaced polymerase chain reaction (TAIL-PCR) is a powerful technique for amplifying genomic regions flanking Tnt1-retortransposon insertions in plants. Here, we present a TAIL-PCR protocol for amplifying Tnt1-flanking genomic sequences in chickpea using Tnt1-transformed hairy roots as the starting material. The amplified products can be cloned and sequenced for the precise mapping of Tnt1-integration sites in the chickpea genome. This method enables the functional characterization of chickpea genes governing root-specific traits and can be easily adapted for flanking sequence tag recovery in chickpea Tnt1-mutant populations.
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    Biotechnological interventions for production of therapeutic secondary metabolites using hairy root cultures of medicinal plants
    (Elsevier B.V., 2016) Srivastava, V.; Mehrotra, S.; Verma, Praveen K.
    The repository of molecules (secondary metabolites) in plants have been identified as an effective candidate to cure human diseases. This immense potential is continuously being utilized by exploiting natural plant resources. Such practices led to environmental imbalances and many natural sites were deserted. This chapter provides current perspectives on plant hairy root cultures as an important model for sustainable therapeutic secondary metabolite production. Here, the imperative interventions of biotechnology to enhance the accumulation of such molecules are discussed. Current research trends for metabolite enhancement through media optimization, elicitation, precursor feeding, metabolic engineering, and upscaling are taken into consideration.