Publications of NIPGR Scientists

Permanent URI for this communityhttps://ndkr-library.nipgr.ac.in/handle/123456789/1

Browse

Subject: search.filters.subject.Bioremediation

Search Results

Now showing 1 - 2 of 2
  • Thumbnail Image
    Item
    Chlorpyrifos degradation by Zhihengliuella sp. ISTPL4: An esterase-driven actinobacterial platform for organophosphorus bioremediation
    (Elsevier B.V., 2026) Aggarwal, Himanshi; Chaudhary, Divya; Kumari, Taruna; Pradhan, Nischal; Mishra, Vaibhav; Kumar, Antresh; Singh, Anamika; Pandey, Ashutosh; Chaturvedi, Navaneet; Dufossé, Laurent; Mishra, Arti; Joshi, Naveen Chandra
    Organophosphorus pesticides (OPs) are widely used agrochemicals that pose serious risks to the environmental and human health due to their persistence and toxicity. This study reports, for the first time, chlorpyrifos (CPF) degradation by actinobacterium Zhihengliuella sp. ISTPL4. Strain ISTPL4 utilized various OPs, including dimethoate, monocrotophos, CPF, and malathion, with the highest growth observed in the presence of CPF as the sole carbon and energy source. Optimal growth and degradation occurred at 28 °C, pH 5, and 3% inoculum in minimal salt medium (MSM). Under optimized conditions, strain ISTPL4 degraded 76.95% of 600 mg L-1 CPF within 7 days. GC-MS analysis identified benzene, 1,3-bis(1,1-dimethylethyl) and phenol, 2,4-bis(1,1-dimethylethyl) as intermediates without the formation of toxic metabolite 3,5,6-trichloro-2-pyridinol (TCP). Whole genome analysis revealed five putative esterase genes potentially associated with CPF degradation. Molecular docking identified carboxylesterase B as the most favorable CPF-binding enzyme, while molecular dynamics simulations supported the stability of the enzyme-substrate complex. A putative metabolic pathway for CPF degradation by strain ISTPL4 was proposed. These findings highlight the potential of Zhihengliuella sp. ISTPL4 as a promising candidate for sustainable bioremediation of OP-contaminated environments.
  • Thumbnail Image
    Item
    Differential expression of peroxidase and ABC transporter as the key regulatory components for degradation of azo dyes by Penicillium oxalicum SAR-3
    (Springer, 2014) Saroj, Samta; Kumar, Karunesh; Prasad, Manoj; Singh, R. P.
    Fungal species are potential dye decomposers since these secrete spectra of extracellular enzymes involved in catabolism. However, cellular mechanisms underlying azo dye catalysis and detoxification are incompletely understood and obscure. A potential strain designated as Penicillium oxalicum SAR-3 demonstrated broad-spectrum catabolic ability of different azo dyes. A forward suppression subtractive hybridization (SSH) cDNA library of P. oxalicum SAR-3 constructed in presence and absence of azo dye Acid Red 183 resulted in identification of 183 unique expressed sequence tags (ESTs) which were functionally classified into 12 functional categories. A number of novel genes that affect specifically organic azo dye degradation were discovered. Although the ABC transporters and peroxidases emerged as prominent hot spot for azo dye detoxification, we also identified a number of proteins that are more proximally related to stress-responsive gene expression. Majority of the ESTs (29.5%) were grouped as hypothetical/unknown indicating the presence of putatively novel genes. Analysis of few ESTs through quantitative real-time reverse transcription polymerase chain reaction revealed their possible role in AR183 degradation. The ESTs identified in the SSH library provide a novel insight on the transcripts that are expressed in P. oxalicum strain SAR-3 in response to AR183.