Publications of NIPGR Scientists

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Subject: search.filters.subject.Abiotic Stresses

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    Current understanding of regulation of GBF3 under abiotic and biotic stresses and its potential role in combined stress tolerance
    (Taylor & Francis Group, 2019) Dixit, Sandeep Kumar; Gupta, Aarti; Senthil-Kumar, Muthappa
    G-box binding factors (GBFs) belong to basic leucine zipper (bZIP) super family of transcription factors. Among six reported members of Arabidopsis thaliana GBFs, AtGBF3 transcripts has been shown to accumulate to high levels in dry seed, stamens, mature pollen and siliques. Amino acid sequence analysis of AtGBF3 reveals presence of proline rich region at N-terminus and basic leucine zipper domain at C-terminus. Earlier, it has been demonstrated that GBFs bind to the G-box element found within the promoter of stress responsive genes including alcohol dehydrogenase (AtAdh) and activate its transcription. Expression profile of AtGBF3 depicts increase in mRNA levels in plants under drought, osmotic, heat, salt and cold stress and biotic stresses. In silico analysis reveals GBF3 localization in nucleus and cytoplasm. Cues from localization studies on GBF3 homologs suggest that upon stress signal perception, GBF3 is phosphorylated and localized to nucleus where it can regulate expression of stress related genes. Till now target genes of GBF3 and mechanism of their regulation during individual and combined abiotic - biotic stresses is not comprehensively reviewed. Through our analysis in this review, we propose GBF3 as major player in integrating abscisic acid and salicylic acid cross talk during combined stress.
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    Comprehensive genomic analysis and expression profiling of phospholipase C gene family during abiotic stresses and development in rice
    (PLOS, 2013) Singh, Amarjeet; Kanwar, Poonam; Pandey, Amita; Tyagi, Akhilesh K.; Sopory, Sudhir K.; Kapoor, Sanjay; Pandey, Girdhar K.
    BACKGROUND: Phospholipase C (PLC) is one of the major lipid hydrolysing enzymes, implicated in lipid mediated signaling. PLCs have been found to play a significant role in abiotic stress triggered signaling and developmental processes in various plant species. Genome wide identification and expression analysis have been carried out for this gene family in Arabidopsis, yet not much has been accomplished in crop plant rice. METHODOLOGY/PRINCIPAL FINDINGS: An exhaustive in-silico exploration of rice genome using various online databases and tools resulted in the identification of nine PLC encoding genes. Based on sequence, motif and phylogenetic analysis rice PLC gene family could be divided into phosphatidylinositol-specific PLCs (PI-PLCs) and phosphatidylcholine- PLCs (PC-PLC or NPC) classes with four and five members, respectively. A comparative analysis revealed that PLCs are conserved in Arabidopsis (dicots) and rice (monocot) at gene structure and protein level but they might have evolved through a separate evolutionary path. Transcript profiling using gene chip microarray and quantitative RT-PCR showed that most of the PLC members expressed significantly and differentially under abiotic stresses (salt, cold and drought) and during various developmental stages with condition/stage specific and overlapping expression. This finding suggested an important role of different rice PLC members in abiotic stress triggered signaling and plant development, which was also supported by the presence of relevant cis-regulatory elements in their promoters. Sub-cellular localization of few selected PLC members in Nicotiana benthamiana and onion epidermal cells has provided a clue about their site of action and functional behaviour. CONCLUSION/SIGNIFICANCE: The genome wide identification, structural and expression analysis and knowledge of sub-cellular localization of PLC gene family envisage the functional characterization of these genes in crop plants in near future.