Publications of NIPGR Scientists

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    Isolation and structural studies of mitochondria from pea roots
    (Springer, 2017) Vishwakarma, Abhaypratap; Gupta, Kapuganti Jagadis
    For structural and respiratory studies, isolation of intact and active mitochondria is essential. Here, we describe an isolation method which gave good yield and intact mitochondria from 2-week-old pea (Pisum sativum) roots grown hydroponically under standard growth conditions. We used Percoll gradient centrifugation for this isolation procedure. The yield of purified mitochondria was 50 μg/g FW. Isolated mitochondria maintained their structure which was observed by using MitoTracker green in confocal microscope and scanning electron microscopy (SEM). Intact mitochondria are clearly visible in SCM images. Taken together this isolation method can be used for physiological and microscopic studies on mitochondria.
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    Regulation of TOP2 by various abiotic stresses including cold and salinity in pea and transgenic tobacco plants
    (Oxford University Press, 2005) Hettiarachchi, Gardhi H. C. M.; Reddy, Malireddy K.; Sopory, Sudhir K.; Chattopadhyay, Sudip
    Although abiotic stress affects plant growth and development, demonstration of its direct effect on regulation of the components of the DNA replication machinery is largely unknown. In this work, we demonstrate that the expression of TOP2 (encoding topoisomerase II) is up-regulated by various abiotic stresses including salinity and low temperature, and phytohormones such as ABA (abscisic acid) and SA (salicylic acid). Transgenic studies with different deletion versions of the TOP2 promoter in tobacco define several promoter determinants responsible for specific abiotic stress responsiveness. Taken together, these results demonstrate a direct involvement of stress in the transcriptional regulation of TOP2.