Publications of NIPGR Scientists

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    Rs_MEP1 is required for the pathogenesis of Rhizoctonia solani AG1-IA in plants
    (The American Phytopathological Society (APS), 2026) Pradhan, Amrita; Sahoo, Debashis; Bhati, Vikrant; Kumar, Rahul; Yadav, Rajni; Ghosh, Srayan; Pawar, Prashant Anupama-Mohan; Jha, Gopaljee
    Rhizoctonia solani AGI-IA is a polyphagous necrotrophic fungal pathogen that causes sheath blight disease in rice. Efforts are being made to identify pathogenicity-associated genes in R. solani and modulate them to develop a disease control strategy. Here, we investigate the roles of some predicted pathogenicity-associated genes of R. solani that have previously been reported to be upregulated during infection in rice. The tobacco rattle virus-based host-induced gene silencing of the selected pathogenicity-associated genes revealed that silencing of Rs_MEP1, a zinc-containing Peptidase_M43 domain-metalloprotease, severely compromises R. solani infection in tomato. Moreover, double-stranded RNA-mediated silencing of Rs_MEP1 prevented R. solani infection in rice. The signal sequence trap assay indicated the secretory nature of Rs_MEP1, while the reporter assay suggested its localization in the plant apoplast. Notably, agrobacterium-mediated transient overexpression of Rs_MEP1 induces necrotic cell death responses in plants. We provide evidence that Rs_MEP1 interacts with GH19 family of rice chitinases and potentially modulates their functions. Overall, our study emphasizes that Rs_MEP1 facilitates R. solani in promoting necrotic responses and targets rice GH19 chitinases to impart disease susceptibility in plants.
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    Genetically engineered crops against bacterial and fungal diseases: a war of attrition
    (Elsevier B.V., 2016) Singh, S.K.; Verma, S.; Verma, Praveen K.
    Phytopathogens such as viruses, bacteria, fungi, and oomycetes have emerged as serious threats to agricultural productivity and food security. Advancements in molecular and genomics studies have offered an efficient and precise understanding of plant–microbe interactions. These studies have also shed light on key components of plant defense mechanisms and led to the discovery of resistance and susceptibility genes, antimicrobial proteins, and defense signaling molecules. Genetic engineering has paved the way for developing designer crops against various biotic and abiotic factors to improve crop yield. Current information regarding extensive studies in plant–microbe interactions has been employed to engineer resistant crops against pathogens and pests. Here we discuss strategies involved in producing disease-resistant crops and examples of genetically engineered crops against bacterial and fungal pathogens.