Publications of NIPGR Scientists

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    Assessing cold stress resilience in wild chickpea accessions using physiological, biochemical, and reproductive traits
    (Springer Nature, 2025) Kaur, Sarbjeet; Padhiar, Deeksha; Singh, Mohar; Parida, Swarup K.; Jha, Uday C.; Sharma, Kamal Dev; Prasad, P. V. Vara; Siddique, Kadambot H. M.; Nayyar, Harsh
    Domesticated chickpea (Cicer arietinum L.) exhibits high sensitivity to temperatures below 20/10 °C during its reproductive phase resulting in substantial loss of flowers, pods and crop yields. With the aim to add new sources of cold tolerance and elucidate mechanism of cold-tolerance in wild species of chickpea, the present study evaluated 36 wild accessions of three Cicer species (Cicer judaicum, Cicer pinnatifidum, Cicer reticulatum) at the reproductive stage for yield, and reproductive, physiological and biochemical traits under cold stress (15/7 °C) for two consecutive years. Cluster analysis based on yield-related traits such as pod number, seed weight, and total seed count categorized these accessions as cold-tolerant and cold-sensitive. Six C. judaicum accessions (ILWC 256, ICC 13852, ILWC 263, ILWC 20, ILWC 223, and ILWC 30) were tolerant to cold whereas the remaining ones were cold-sensitive. Under cold stress, cold-tolerant accessions exhibited lower impairment of physiological processes as compared to the cold-sensitive accessions e.g. lower tissue damage and electrolyte leakage, and higher chlorophyll content, carotenoid content, chlorophyll fluorescence, and leaf water content, thereby resulting in higher photosynthetic efficiency and carbohydrate accumulation in cold-tolerant accessions. At the biochemical level, the tolerant accessions demonstrated significantly higher amounts of cryoprotectants and enhanced activities of enzymatic and non-enzymatic antioxidants resulting in substantially lower levels of reactive oxygen species. Cold-tolerant accessions also accumulated more proline and trehalose compared to their sensitive counterparts. Slight disruptions in physiological processes, low oxidative stress and accumulation of cryoprotectants under cold stress were associated with higher pollen viability, pollen germination, pollen load, ovule receptivity, pod set, number of pods and seed yield in cold-tolerant accessions while opposite was true for cold-sensitive accessions. The wild chickpea accessions exhibiting high seed yield under cold stress are promising candidates for breeding programs aimed at cold tolerance.
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    Genome-wide identification, characterization, and expression analysis of Clavata3 Insensitive receptor Kinases (CIKs) on developmental tissues and under temperature stress conditions in Brassica oleracea
    (Springer Nature Publishing AG, 2025) Suresh, Gokul Babu; Baskar, Harssitha; Subramaniam, Geethanjali; Soundararajan, Prabhakaran
    Unprecedented climate changes are one of the major global threats to agriculture. Though cauliflower is grown worldwide in different conditions, it is sensitive to temperature fluctuation. Clavata3 Insensitive receptor Kinase (CIK) is one of the important co-receptors involved in WUS-CLV pathway for shoot apical meristem (SAM) maintenance, floral development and environment buffering. In this study we did genome-wide identification and characterization of CIKs in Brassica oleracea var. botrytis cv. Korso. Expression analysis was conducted on developmental tissues and short-term heat and cold stress conditions. Seven fulllength BolCIKs have been identified in the Korso genome through comparison against Arabidopsis thaliana CIKs. Gene structure and motifs are distinct between each sub-class. Ka/Ks ratios showed that all CIKs underwent purifying selection. Abundance of stress-responsive cis-elements, such as MYB and MYC recognition sites, showed the involvement of BolCIK in environmental stress regulation. Among the nine developmental tissues such as leaf, stem, root, curd, sepal, petal, stamen, pistil, and silique, except BolCIK4 and BolCIK5a, CIKs were merely detected in leaves. Contrastingly, expression of all BolCIKs was detected in stem, curd, and reproductive organs. Importantly, most of the BolCIKs were highly expressed in sepal, petal, pistil, and siliques. In SAM specific manner, BolCIK3 and BolCIK6 are upregulated in heat stress and cold stress, respectively. Other BolCIKs expressions are varying between tissues-types and stress conditions. Since CIKs can possibly be involved in thermomorphogenesis, results of this work may lead to further exploration of its function to improve climate resilience in cauliflower.
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    New evidences about strictosidine synthase (Str) regulation by salinity, cold stress and nitric oxide in Catharanthus roseus
    (Springer, 2013) Dutta, Ajaswrata; Sen, Jayanti; Deswal, Renu
    Alkaloid production in plants is altered by abiotic stressors, but the mechanism(s) are poorly understood. Present study provides novel evidences about differential regulation of strictosidine synthase (Str), the key gene of terpenoid indole alkaloid (TIA) biosynthetic pathway in response to salinity and low temperature stress in Catharanthus roseus. HPLC analysis of terpene indole alkaloids correlated with differential regulation of Str by low temperature and salinity stress. Administration of exogenous calcium and calcium channel modulator preferentially regulated Str transcript. In addition, administration of kinase and phosphatase inhibitors modulated Str expression. Involvement of nitric oxide (NO) signaling was ascertained by NO donor and nitric oxide synthase (NOS) scavenger treatments. This finding suggests co-ordinated action of calcium, protein kinases, phosphatases and nitric oxide in abiotic stress signaling for TIA biosynthetic pathway in C. roseus.