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Start date: 2022Subject: search.filters.subject.Jasmonate

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    Quantification of jasmonic acid and salicylic acid in plant-insect interactions
    (Springer Nature Publishing AG, 2026) Meena, Mukesh Kumar
    Plants encounter a variety of insect herbivores and have developed complex defense mechanisms against different feeding strategies. Phytohormones jasmonic acid (JA) and salicylic acid (SA) play a central role in recognizing and mounting appropriate defense response against pathogens. Primarily, JA provides resistance against chewing insect herbivores, whereas SA bolster defense against biotrophs and phloem-feeding insects (aphids and whitefly). Complex interaction of JA and SA signaling creates a plant stress memory which helps to generate elevated defense response to future insect threats. Phytohormone quantification can provide significant insights about activated plant defense response and involved signaling pathways. Here, we describe a detailed and reproducible protocol for phytohormone quantification (SA, ABA, JA, and JA-Ile) in insect-fed Arabidopsis leaves tissues. JA and JA-Ile quantification results clearly indicate their contribution in plant adaptation and defense response.
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    Jasmonic acid coordinates with light, glucose and auxin signalling in regulating branching angle of Arabidopsis lateral roots
    (John Wiley & Sons, 2022) Sharma, Manvi; Sharma, Mohan; Jamsheer, K. Muhammed; Laxmi, Ashverya
    The role of jasmonates (JAs) in primary root growth and development and in plant response to external stimuli is already known. However, its role in lateral root (LR) development remains to be explored. Our work identified methyl jasmonate (MeJA) as a key phytohormone in determining the branching angle of Arabidopsis LRs. MeJA inclines the LRs to a more vertical orientation, which was dependent on the canonical JAR1-COI1-MYC2,3,4 signalling. Our work also highlights the dual roles of light in governing LR angle. Light signalling enhances JA biosynthesis, leading to erect root architecture; whereas, glucose (Glc) induces wider branching angles. Combining physiological and molecular assays, we revealed that Glc antagonizes the MeJA response via TARGET OF RAPAMYCIN (TOR) signalling. Moreover, physiological assays using auxin mutants, MYC2-mediated transcriptional activation of LAZY2, LAZY4 and auxin biosynthetic gene CYP79B2,and asymmetric distribution of DR5::GFP and PIN2::GFP pinpointed the role of an intact auxin mechanism required by MeJA for vertical growth of LRs. We also demonstrated that light perception and signalling are indispensable for inducing vertical angles by MeJA. Thus, our investigation highlights antagonism between light and Glc signalling and how they interact with JA-auxin signals to optimize the branching angle of LRs.