Publications of NIPGR Scientists

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Author: search.filters.author.Singh, KashmirSubject: search.filters.subject.metabolites

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    Targeted metabolite profiling of five cultivars of Vitis vinifera L. fruits
    (American Chemical Society, 2021) Alok, Anshu; Tyagi, Shivi; Singh, Kashmir; Pandey, Ashutosh
    Grapes (Vitis vinifera L.) are a very popular fruit and are produced around the world. They are consumed as a fresh ripe fruit, dried fruit, juice, and processed product such as wine and are a good source of human nutrition. We aimed to investigate and analyze the nutritional and phytochemical components of grape cultivars, mostly cultivated in different regions of India. The targeted metabolic profiling of these cultivars was examined. A total of 58 metabolites, including three major sugars, nine organic acids, 14 amino acids, nine vitamins, and 23 phenolic compounds, were quantified in ripe berries of these grapes. Mineral quantification showed that these varieties contain major elements (K, Mg, and Ca) and minor elements (Mn, Fe, Zn, Cu, and Na). Principal component analysis showed that the contents of sugars, organic acids, and phenolic compounds are almost similar in cultivars FS and BJ, whereas the vitamin contents are nearly similar in TS and GS. Overall, these findings reveal that there is a differential in the accumulated content of primary and specialized metabolites as well as minerals in ripe fruits, providing a source for commercial value and food industry applications.
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    An RNA isolation system for plant tissues rich in secondary metabolites
    (BioMed Central, 2011) Ghawana, Sanjay; Paul, Asosii; Kumar, Hitesh; Kumar, Arun; Singh, Harsharan; Bhardwaj, Pardeep K; Rani, Arti; Singh, Ravi S; Raizada, Jyoti; Singh, Kashmir; Kumar, Sanjay
    Secondary metabolites are reported to interfere with the isolation of RNA particularly with the recipes that use guanidinium-based salt. Such interference was observed in isolation of RNA with medicinal plants rheum (Rheum australe) and arnebia (Arnebia euchroma). A rapid and less cumbersome system for isolation of RNA was essential to facilitate any study related to gene expression.