Publications of NIPGR Scientists
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Item Histone deacetylase 9 interacts with SiHAT3.1 and SiHDA19 to repress dehydration responses through H3K9 deacetylation in foxtail millet(Oxford University Press, 2024) Kumar, Verandra; Singh, Babita; Singh, Roshan Kumar; Sharma, Namisha; Muthamilarasan, Mehanathan; Sawant, Samir V; Prasad, ManojClimate change inflicts several stresses on plants, of which dehydration stress severely affects growth and productivity. C4 plants possess better adaptability to dehydration stress; however, the role of epigenetic modifications underlying this trait is unclear. Particularly, the molecular links between histone modifiers and their regulation remain elusive. In this study, genome-wide H3K9 acetylation (H3K9ac) enrichment using ChIP-seq was performed in two foxtail millet cultivars contrastingly differing in dehydration tolerance (IC403579; cv. IC4 – tolerant, and IC480117; cv. IC41 – sensitive). It revealed that a histone deacetylase, SiHDA9, was significantly up-regulated in the sensitive cultivar. Further characterization indicated that SiHDA9 interacts with SiHAT3.1 and SiHDA19 to form a repressor complex. SiHDA9 might be recruited through the SiHAT3.1 recognition sequence onto the upstream of dehydration-responsive genes to decrease H3K9 acetylation levels. The silencing of SiHDA9 resulted in the up-regulation of crucial genes, namely, SiRAB18, SiRAP2.4, SiP5CS2, SiRD22, SiPIP1;4 and SiLHCB2.3, which imparted dehydration tolerance in the sensitive cultivar (IC41). Overall, the study provides mechanistic insights into SiHDA9-mediated regulation of dehydration stress response in foxtail millet.Item Chromatin-based epigenetic regulation of plant abiotic stress response(Bentham Science, 2016) Pandey, Garima; Sharma, Namisha; Sahu, Pranav Pankaj; Prasad, ManojPlants are continuously exposed to various abiotic and biotic factors limiting their growth and reproduction. In response, they need various sophisticated ways to adapt to adverse environmental conditions without compromising their proper development, reproductive success and eventually survival. This requires an intricate network to regulate gene expression at transcriptional and post-transcriptional levels, including epigenetic switches. Changes in chromatin modifications such as DNA and histone methylation have been observed in plants upon exposure to several abiotic stresses. In the present review, we highlight the changes of DNA methylation in diverse plants in response to several abiotic stresses such as salinity, drought, cold and heat. We also discuss the progresses made in understanding how these DNA methylation changes might contribute to the abiotic stress tolerance.Item Role of NACs in regulation of abiotic stress responses in plants(Bentham Science Publishers, USA, 2012) Puranik, Swati; Prasad, ManojAbiotic stresses such as drought, high salinity and cold are common adverse environmental conditions that significantly influence plant growth and productivity worldwide. NAC domain proteins are important plant-specific transcription factors (TFs) that regulate the expression of many stress-inducible genes. They act both by an ABA-dependent or independent manner and play a critical role in improving abiotic stress tolerance of plants by interacting with cis- element present in the promoter region of various abiotic stress- responsive genes. We summarize recent studies highlighting the structural and functional characters of specific members of this family, the current knowledge on the relation between NACs and their cis-elements, with emphasis on the expression and regulation of NACs in the adaptive responses to abiotic stresses. The progress of the practical and application value of NACs in crop improvement engineering has also been discussed