Publications of NIPGR Scientists

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Author: search.filters.author.Pandey, SaurabhHas files: Yes

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Now showing 1 - 7 of 7
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    Catalase regulation during plant-virus-vector interaction
    (John Wiley & Sons, 2024) Sharma, Rohit; Pandey, Saurabh; Prasad, Manoj; Prasad, Ashish
    Plant-virus-host interaction is a complex process involving several players. A constant arms race between the hosts and viruses has led to their co-evolution. Reactive oxygen species (ROS) are important signaling molecules that regulate plant growth, development, and stress responses. Barley yellow dwarf virus (BYDV) has a wide host range and infects several plant species such as barley, rice, oats, wheat, etc. A recent study by Tian et al. (2024) has highlighted that the movement protein (MP) of BYDV is involved in manipulation of the host ROS pathway to promote viral multiplication as well as transmission. The findings display the multifaceted role of a viral protein that is otherwise involved in movement. The limited coding ability of viruses is compensated by their proteins having multiple roles in the modulation of several different host molecular pathways. This is one of the key reasons for viruses being successful pathogens despite their limited coding ability.
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    Combining speed breeding with traditional and genomics-assisted breeding for crop improvement
    (John Wiley & Sons, 2022) Pandey, Saurabh; Singh, Ashutosh; Parida, Swarup K.; Prasad, Manoj
    Accelerated crop growth strategy innovations are required as we reach saturation peaks regarding the productivity of major food crops. Speed breeding (SB) is one of the most promising technologies adopted for this purpose. SB hastens crop production by reducing plant growth and development, breeding time and swift generation advancement. Prolonged daily light exposure shortens the life cycle in some long-day or day-neutral plants leading to early seed harvest. This approach is best suited for controlled environment prebreeding/breeding activities and analysed for several crop species. SB can be integrated with different traditional and advanced genomics-assisted breeding technologies like marker-assisted selection (MAS), genomic selection (GS), pollen-based selection (PBS), overexpression/knock-down transgenics and genome editing to achieve more precise and faster results on translational genetic enhancement. This review will discuss the approaches and strategies adopted for the SB and its potential to integrate existing crop improvement technologies to attain more efficient outcomes on major food crops' varietal improvement.
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    Linking the plant stress responses with RNA helicases
    (Elsevier B.V., 2020) Pandey, Saurabh; Prasad, Ashish; Sharma, Namisha; Prasad, Manoj
    RNA helicases are omnipresent plant proteins across all kingdoms and have been demonstrated to play an essential role in all cellular processes involving nucleic acids. Currently, these proteins emerged as a new tool for plant molecular biologists to modulate plant stress responses. Here, we review the crucial role of RNA helicases triggered by biotic, abiotic, and multiple stress conditions. In this review, the emphasis has been given on the role of these proteins upon viral stress. Further, we have explored RNA helicase mediated regulation of RNA metabolism, starting from ribosome biogenesis to its decay upon stress induction. We also highlighted the cross-talk between RNA helicase, phytohormones, and ROS. Different overexpression and transgenic studies have been provided in the text to indicate the stress tolerance abilities of these proteins.
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    Role of RNA-interacting proteins in modulating plant-microbe interactions
    (Elsevier B.V., 2020) Pandey, Saurabh; Sharma, Namisha; Prasad, Manoj
    Successful infection of a pathogen in its host plant depends on the complex molecular interplay between host and the invading microbe. Plant-microbe interactions are primarily governed by signal interchange amid both the organisms. Effective passage of the pathogen into the plant system requires the circumvention of signal detection mechanisms and subsequent immune responses. As a mechanism to counteract defense response, pathogens deploy several RNA-interacting proteins (RIPs) or RNA molecules which interrupt the host transcriptional as well as signaling pathways, leading to successful infection and symptom development. The interference by pathogen-derived small RNAs (sRNA) in the gene silencing machinery of the host has been recently reported. In this context, the present review describes the pathogen- and host-specific RNA molecules and chaperones, their roles in modulating host immune response as well as pathogenesis, and the possible targets for manipulating the molecular mechanism to develop durable tolerance/resistance against diseases in crops.
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    Characterization of DEAD-box family of RNA helicases in tomato provides insights into their roles in biotic and abiotic stresses
    (Elsevier B.V., 2019) Pandey, Saurabh; Muthamilarasan, Mehanathan; Sharma, Namisha; Chaudhry, Vaishali; Dulani, Priya; Shweta, Shweta; Jha, Sarita; Mathur, Saloni; Prasad, Manoj
    In plants, RNA helicases play significant roles in growth, development and stress response. In a previous study, a three-fold upregulation of a DEAD-box RNA helicase in a tomato cultivar tolerant to Tomato leaf curl New Delhi virus (ToLCNDV) as compared to susceptible cultivar during virus infection was shown. Given this, a comprehensive study was performed to identify the members of RNA helicase family in tomato and analyze their functional properties in response to abiotic stresses, hormone treatments and ToLCNDV infection. A total of 131 genes were identified and classified into DEAD- (42), DEAH- (38), and DExD/H-box (51) RNA helicases. Expression profiling of candidate genes in response to abiotic stresses and ToLCNDV infection in contrasting tomato cultivars suggested the putative roles of SlDEAD23 and SlDEAD35 in biotic and abiotic stresses. Heterologous overexpression of these genes in yeast enhanced the tolerance of transgenic cells to salt and cold stresses. Further, virus-induced silencing of SlDEAD35 in ToLCNDV tolerant cultivar resulted in susceptibility to virus infection, thus suggesting its involvement in tolerance mechanism. Altogether, this study provides novel insights into the structure, organization and involvement of DEAD-box RNA helicase genes in biotic and abiotic stress responses in tomato.
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    Role of host transcription factors in modulating defense response during plant-virus interaction
    (Caister Academic Press, 2018) Pandey, Saurabh; Sahu, Pranav P.; Kulshreshtha, Ritika; Prasad, Manoj
    Plants are vulnerable to several environmental stresses either biotic or abiotic due to their sessile nature. Consequently, they assimilate various responses to counter and acclimate in ever-changing environments. One of the fascinating response is transcriptional reprogramming of cell leading to defense or stress adaptation. It is imperative to recognize transcription factors which are associated with plant defense responses against biotic agents such as viruses. Members of families belonging to WRKY family of transcription factor, myeloblastosis-related proteins (MYB), basic leucine zipper (bZIP), Apetela2/ethylene-responsive element binding (AP2/ERF), and NAC transcription factors have been shown to be associated with innumerable defense response against plant virus. These TF family members interact directly or indirectly to modulate defense response by activation or repression of downstream signaling pathways. Hence the gaining insight between plant virus and TFs interplay and deciphering the alterations in defense pathway are the prerequisite to engineer crops for tolerance to biotic stresses. In this chapter, we have attempted to summarize the explicit role of TFs in modulating the expression of defense genes during plant-virus interaction.
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    Genomics resources for abiotic stress tolerance in solanaceae crops
    (Springer, 2017) Sharma, Shambhavi; Pandey, Saurabh; Muthamilarasan, Mehanathan; Chaudhry, Vaishali; Dulani, Priya; Prasad, Manoj
    The study of abiotic stresses in plants is crucial for an understanding of the mechanisms involved in responses to these stresses. The complex nature of abiotic stress-related traits and the occurrence of more than one stress simultaneously further complicate the study. The availability of genomic tools and resources allows a leap in plant breeding by facilitating the study of the genotype and its relationship with the phenotype. The development of techniques such as Next-Generation Sequencing (NGS) allowed the sequencing of genomes of cultivars and their wild relatives, enriching the available genetic as well as genomic resources. Genome-wide discovery of markers and quantitative trait loci are used for marker-assisted selection and breeding. The availability of the genome sequence information has expedited several downstream analyses, including genome-wide identification and expression profiling of the genes associated with stress response. This is coupled with the use of mutants and transgenics to elucidate and verify the function of genes in a high-throughput fashion. In this chapter, the progress made in the generation and enrichment of genomic resources of Solanum tuberosum and S. lycopersicum are discussed from the point of view of genetic improvement for abiotic stress tolerance.