Publications of NIPGR Scientists

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Author: search.filters.author.Mysore, Kirankumar S.Has files: No

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    Recent advances in plant gene silencing methods
    (Springer Nature Publishing AG, 2022) Pandey, Prachi; Mysore, Kirankumar S.; Senthil-Kumar, Muthappa
    With the increasing understanding of fundamentals of gene silencing pathways in plants, various tools and techniques for downregulating the expression of a target gene have been developed across multiple plant species. This chapter provides an insight into the molecular mechanisms of gene silencing and highlights the advancements in various gene silencing approaches. The prominent aspects of different gene silencing methods, their advantages and disadvantages have been discussed. A succinct discussion on the newly emerged microRNA-based technologies like microRNA-induced gene silencing (MIGS) and microRNA-mediated virus-induced gene silencing (MIR-VIGS) are also presented. We have also discussed the gene-editing system like CRISPR-Cas. The prominent bottlenecks in gene silencing methods are the off-target effects and lack of universal applicability. However, the tremendous growth in understanding of this field reflects the potentials for improvements in the currently available approaches and the development of new widely applicable methods for easy, fast, and efficient functional characterization of plant genes.
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    High-throughput analysis of gene function under multiple abiotic stresses using leaf disks from silenced plants
    (Springer Nature Publishing AG, 2022) Yamunarani, Ramegowda; Ramegowda, Venkategowda; Senthil-Kumar, Muthappa; Mysore, Kirankumar S.
    The high throughputness and affordability of “omics” technologies is leading to the identification of a large number of abiotic stress genes, with many of them responsive to multiple stresses. In vivo functional characterization of these genes under multiple stresses is challenging but essential to develop resilient crops for the changing climate. Here we describe a high-throughput Virus-Induced Gene Silencing-based methodology for functional analysis of genes under multiple abiotic stresses using leaf disks. Leaves with maximal silencing, which is localized to only a few leaves and to a short period, can be effectively used for multiple stress imposition and stress affect quantification.