Publications of NIPGR Scientists

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Author: search.filters.author.Mithöfer, AxelSubject: search.filters.subject.Jasmonate

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    Omega hydroxylated JA-Ile is an endogenous bioactive jasmonate that signals through the canonical jasmonate signaling pathway
    (Elsevier B.V., 2019) Jimenez-Aleman, Guillermo H.; Almeida-Trapp, Marilia; Fernández-Barbero, Gemma; Gimenez-Ibanez, Selena; Reichelt, Michael; Vadassery, Jyothilakshmi; Mithöfer, Axel; Caballero, Julio; Boland, Wilhelm; Solano, Roberto
    Jasmonates are fatty acid derivatives that control several plant processes including growth, development and defense. Despite the chemical diversity of jasmonates, only jasmonoyl-L-isoleucine (JA-Ile) has been clearly characterized as the endogenous ligand of the jasmonate co-receptors (COI1-JAZs) in higher plants. Currently, it is accepted that ω-hydroxylation of JA-Ile leads to inactivation of the molecule. This study shows that ω-hydroxylated JA-Ile (12-OH-JA-Ile) retains bioactivity and signals through the canonical JA-pathway. The results suggest that 12-OH-JA-Ile differentially activates a subset of JA-Ile co-receptors that may control and/or modulate particular jasmonate dependent responses. It is proposed that after a strong immune response mediated by JA-Ile, the ω-hydroxylated form modulates JA-Ile activated processes thereby improving plant resilience.
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    Neomycin: an effective inhibitor of jasmonate-induced reactions in plants
    (Springer Nature, 2019) Vadassery, Jyothilakshmi; Ballhorn, Daniel J.; Fleming, Steven R.; Mazars, Christian; Pandey, Shree P.; Schmidt, Axel; Schuman, Meredith C.; Yeh, Kai‑Wun; Yilamujiang, Ayufu; Mithöfer, Axel
    Jasmonates are important phytohormones involved in both plant developmental processes as well as defense reactions. Many JA-mediated plant defense responses have been studied in model plants using mutants of the jasmonate signaling pathway. However, in plant species where JA-signaling mutants are not accessible, the availability of a tool targeting JA signaling is crucial to investigate jasmonate-dependent processes. Neomycin is a poly-cationic aminoglycoside antibiotic that blocks the release of Ca2+ from internal stores. We examined the inhibitory activities of neomycin on different jasmonate-inducible responses in eight different plant species: Intracellular calcium measurements in Nicotiana tabacum cell culture, Sporamin gene induction in Ipomoea batatas, PDF2.2 gene expression in Triticum aestivum, Nepenthesin protease activity measurement in Nepenthes alata, extrafloral nectar production in Phaseolus lunatus, nectary formation in Populus trichocarpa, terpene accumulation in Picea abies, and secondary metabolite generation in Nicotiana attenuata. We are able to show that neomycin, an easily manageable and commercially available compound, inhibits JA-mediated responses across the plant kingdom.