Publications of NIPGR Scientists

Permanent URI for this communityhttps://ndkr-library.nipgr.ac.in/handle/123456789/1

Browse

Filters

20132

Settings

Author: search.filters.author.Chattopadhyay, DebasisSubject: search.filters.subject.Stress Tolerance

Search Results

Now showing 1 - 2 of 2
  • Thumbnail Image
    Item
    Role of DREB-Like proteins in improving stress tolerance of transgenic crops
    (Springer, 2013) Jain, Deepti; Chattopadhyay, Debasis
    How crop plants respond to stresses in their physical environment is crucial to their productivity. Of all parameters, abiotic environmental stresses contribute most significantly to the reduction in potential yield. Plants respond to different forms of abiotic stresses by inducing common and distinct set of genes in abscisic acid (ABA)-dependent and independent manner. These genes can be divided in two major classes, genes encoding functional proteins and genes encoding regulatory proteins. Because of practical limitations of expressing multiple genes in a plant in a tissue specific manner, genes that can regulate a number of functionally related genes can be attractive targets for genetic manipulation. Therefore, transcription factors operating very early in the stress response pathways before accumulation of ABA would be potential candidates for engineering stress tolerance. Studies in last decade have focused on such a group of transcription factors known as dehydration-responsive element-binding proteins (DREB) those functions in ABA-independent manner very early in stress response pathway. In recent years a group of small DREB-like proteins has emerged that shows ABA-responsiveness unlike the authentic DREB proteins. These proteins when ectopically expressed do not show any growth penalty in contrast to DREB-expressing transgenics. However, these genes impart stress tolerance to the expressing plants. All these observations are changing the decade-long concepts on functioning of DREB proteins and widening scope of research on this agronomically important family.
  • Thumbnail Image
    Item
    Promoter of CaZF, a chickpea gene that positively regulates growth and stress tolerance, is activated by an AP2-family transcription factor CAP2
    (PLOS, 2013) Jain, Deepti; Chattopadhyay, Debasis
    Plants respond to different forms of stresses by inducing transcription of a common and distinct set of genes by concerted actions of a cascade of transcription regulators. We previously reported that a gene, CaZF encoding a C2H2-zinc finger family protein from chickpea (Cicer arietinum) imparted high salinity tolerance when expressed in tobacco plants. We report here that in addition to promoting tolerance against dehydration, salinity and high temperature, the CaZF overexpressing plants exhibited similar phenotype of growth and development like the plants overexpressing CAP2, encoding an AP2-family transcription factor from chickpea. To investigate any relationship between these two genes, we performed gene expression analysis in the overexpressing plants, promoter-reporter analysis and chromatin immunoprecipitation. A number of transcripts that exhibited enhanced accumulation upon expression of CAP2 or CaZF in tobacco plants were found common. Transient expression of CAP2 in chickpea leaves resulted in increased accumulation of CaZF transcript. Gel mobility shift and transient promoter-reporter assays suggested that CAP2 activates CaZF promoter by interacting with C-repeat elements (CRTs) in CaZF promoter. Chromatin immunoprecipitation (ChIP) assay demonstrated an in vivo interaction of CAP2 protein with CaZF promoter.