Publications of NIPGR Scientists

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Author: search.filters.author.Chakraborty, SubhraSubject: search.filters.subject.Proteomics

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    Plant fungus interaction proteomics: An update
    (Springer, 2016) Narula, Kanika; Aggarwal, Pooja R.; Chakraborty, Niranjan; Chakraborty, Subhra
    Diversity of angiosperm is renowned and mechanism of perception and interaction with different environmental conditions is also variable. Patho-stress response in different plant families varies during the invasion of same or different fungal species. A major puzzle is how interaction and communication could increase fitness in plant at molecular level. Global proteome analysis of plant-pathosystem provides an invaluable resource for the identification of host as well as pathogen proteins involved in disease progression or immunity development. At protein level plant-fungal interaction upsurge the need to understand protein homeostasis and molecular adaptation of building blocks of cell to manifest natural selection for the host. Here, we examine the multilayered facets of interaction between organisms of two diverse kingdoms, namely plant and fungi at protein level based on more than 3000 identified host proteins till date.
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    Proteomics approach to identify dehydration responsive nuclear proteins from chickpea (Cicer arietinum L.)
    (The American Society for Biochemistry and Molecular Biology, Inc., 2008) Pandey, Aarti; Chakraborty, Subhra; Datta, Asis; Chakraborty, Niranjan
    Dehydration or water-deficit is one of the most important environmental stress factors that greatly influences plant growth and development and limits crop productivity. Plants respond and adapt to such stress by altering their cellular metabolism and activating various defense machineries. Mechanisms that operate signal perception, transduction, and downstream regulatory events provide valuable information about the underlying pathways involved in environmental stress responses. The nuclear proteins constitute a highly organized, complex network that plays diverse roles during cellular development and other physiological processes. To gain a better understanding of dehydration response in plants, we have developed a comparative nuclear proteome in a food legume, chickpea (Cicer arietinum L.). Three-week-old chickpea seedlings were subjected to progressive dehydration by withdrawing water and the changes in the nuclear proteome were examined using two-dimensional gel electrophoresis. Approximately 205 protein spots were found to be differentially regulated under dehydration. Mass spectrometry analysis allowed the identification of 147 differentially expressed proteins, presumably involved in a variety of functions including gene transcription and replication, molecular chaperones, cell signaling, and chromatin remodeling. The dehydration responsive nuclear proteome of chickpea revealed a coordinated response, which involves both the regulatory as well as the functional proteins. This study, for the first time, provides an insight into the complex metabolic network operating in the nucleus during dehydration.