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    PtRFdb: Plant tRNA-derived fragments database
    (Springer Nature Publishing AG, 2019) Zahra, Shafaque; Kumar, Shailesh
    The transfer RNA-derived fragments or tRFs represent a distinct class of small non-coding RNAs, and have been detected in evolutionarily divergent organisms. The role of tRFs in human cancers and infectious diseases as well as in gene regulation has been well established in diverse organisms. However, in plants, there is a need to further consolidate the tRF research by identification and characterization of tRFs because this domain is still unexplored across the plant kingdom. This chapter discusses about PtRFdb (www.nipgr.res.in/PtRFdb), a web-based repository harbouring the valuable information related to transfer RNA derived fragments (tRFs) in 10 different plant species. This database is believed to be beneficial for molecular biologists in facilitating future survey and characterization of tRFs across the plant kingdom.
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    PtRFdb: a database for plant transfer RNA-derived fragments
    (Oxford University Press, 2018) Gupta, Nikita; Singh, Ajeet; Zahra, Shafaque; Kumar, Shailesh
    Transfer RNA-derived fragments (tRFs) represent a novel class of small RNAs (sRNAs) generated through endonucleolytic cleavage of both mature and precursor transfer RNAs (tRNAs). These 14–28 nt length tRFs that have been extensively studied in animal kingdom are to be explored in plants. In this study, we introduce a database of plant tRFs named PtRFdb (www.nipgr.res.in/PtRFdb), for the scientific community. We analyzed a total of 1344 sRNA sequencing datasets of 10 different plant species and identified a total of 5607 unique tRFs (758 tRF-1, 2269 tRF-3 and 2580 tRF-5), represented by 487 765 entries. In PtRFdb, detailed and comprehensive information is available for each tRF entry. Apart from the core information consisting of the tRF type, anticodon, source organism, tissue, sequence and the genomic location; additional information like PubMed identifier (PMID), Sample accession number (GSM), sequence length and frequency relevant to the tRFs may be of high utility to the user. Two different types of search modules (Basic Search and Advanced Search), sequence similarity search (by BLAST) and Browse option with data download facility for each search is provided in this database. We believe that PtRFdb is a unique database of its kind and it will be beneficial in the validation and further characterization of plant tRFs. Database URL: http://www.nipgr.res.in/PtRFdb/