Institutional Publications
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Item Brassinosteroids-regulated nitrogen metabolism fine-tunes growth physiology and low nitrogen response in tomato(Elsevier B.V., 2023) Yadav, Ritesh Kumar; Analin, Benedict; Panda, Mahesh Kumar; Ranjan, Aashish; Singh, Amar PalNitrogen (N) is a crucial nutrient for plants and its limited availability in the soils significantly affects plant growth and development. To adapt under low N condition, plants undergo various changes such as root system reprogramming to explore deeper soil horizons and metabolic activity adjustment. These N dependent responses and the genetic factors governing them are poorly known in crop plants. In this study, we investigated the effect of BRs on N metabolism in tomato. BRs application improved N assimilation and metabolic responses. By using the transgenic approach, we demonstrated the essential role of tomato Brassinazole resistant (BES1/BZR1) homolog 4 (BEH4) protein in regulating N metabolic response, growth physiology, and fruit quality. Overexpression of BEH4 promoted deeper root system architecture and improved physiological performance by adjusting N metabolic activity and photosynthetic efficiency in low N-grown plants. The BEH4 transgenic lines exhibited increased expression of genes involved in N uptake and assimilation which are associated with the improved N content and assimilation (root and shoot). Altogether, data suggested an essential role of BRs in plant adaptation to altered N regimes and appears potential target for genetic manipulation to improve nitrogen use efficiency (NUE) and nutritional quality in crops.Item A precise method for analyzing nitrogen use in foxtail millet(Springer Nature Publishing AG, 2020) Bandyopadhyay, Tirthankar; Prasad, ManojOptimization of biological nitrogen (N) use is instrumental in ensuring higher crop yields and preventing environmental degradation due to excessive N fertilizer application. Furthermore, understanding how genetic differences differentially influence N remobilization into seeds under contrasting nitrogen nutrition regimes is crucial to our understanding of nitrogen use efficiency (NUE) in crops in addition to enabling a deeper mechanistic understanding of the dynamics of nitrogen metabolism in plants. In this chapter, a method is proposed to precisely measure and analyze nitrogen use efficiency (NUE) in a pot-based system under different nitrogen nutrition regimes in foxtail millet (Setaria italica L.), a climate change-resilient C4 model crop with great promise for food security and nutrition in the twenty-first century.
