Institutional Publications

Permanent URI for this collectionhttps://ndkr-library.nipgr.ac.in/handle/123456789/11

Browse

Subject: search.filters.subject.Lipids

Search Results

Now showing 1 - 3 of 3
  • Thumbnail Image
    Item
    Lipid-mediated responses to nutrient and other stresses: Roles in plant adaptation and signaling
    (Oxford University Press, 2026) Pandey, Mandavi; Ganotra, Jahanvi; Singh, Astha; Parchuri, Prasad; Giri, Jitender
    Lipids are essential components of biological membranes; however, their roles in plants go far beyond providing structural support. They are actively involved in signaling and metabolic regulation during abiotic and biotic stress. Plants use lipid-based strategies to adapt to nutrient shortages, toxic conditions, and changing environments. Signaling lipids such as phosphatidic acid (PA), phosphoinositides, sphingolipids, and oxylipins serve as molecular messengers that transmit stress signals to regulate ion transport, hormone interactions, and developmental flexibility. During nutrient deprivation, especially of phosphorus (P), nitrogen (N), and potassium (K), plants adjust membrane composition by replacing phospholipids with P-free glycolipids like galactolipids and sulfolipids, conserving critical nutrients while keeping membrane structure intact. Lipid intermediates, including PA, glycerol-3-phosphate, and negatively charged phospholipids, also act as secondary messengers in stress signaling networks. Lipid droplets and lipophagy help maintain carbon and redox balance, while enzymes like phospholipases and glycerophosphodiesterases recycle nutrients and alter lipid profiles. Under K deficiency and toxic metal exposure (e.g., aluminum (Al), cadmium (Cd)), lipid turnover influences membrane stability, reactive oxygen species (ROS) production, and transporter function. Lipid modifications also reduce toxic ion binding and cellular damage, and molecules derived from lipids, such as jasmonates, play roles in secondary metabolism and hormonal defense pathways. Although lipid-based stress responses are conserved throughout plant lineages, the regulatory mechanisms controlling lipid fluxes are not yet fully understood. New tools, like genetically coded lipid biosensors and lipidomics platforms, are uncovering the spatial and temporal dynamics of lipid signaling with unprecedented detail. Improving our understanding of lipid-mediated stress responses may enable the development of crops with better nutrient efficiency and resilience to climatic and edaphic stresses, contributing to sustainable agriculture.
  • No Thumbnail Available
    Item
    Monogalactosyl Diacylglycerol Synthase 3 affects phosphate utilization and acquisition in rice
    (Oxford University Press, 2022) Verma, Lokesh; Bhadouria, Jyoti; Rupam, Bhunia Kumar; Singh, Shweta; Panchal, Poonam; Bhatia, Chitra; Eastmond, Peter J; Giri, Jitender
    Galactolipids are essential for compensating for the loss of phospholipids by “membrane lipid remodeling” in plants under phosphorus (P) deficiency. Monogalactosyl diacylglycerol (MGDG) synthases catalyze the synthesis of MGDG which is further converted into digalactosyl diacylglycerol (DGDG), later replacing phospholipids in the extraplastidial membranes. However, the roles of these enzymes are not well explored in rice. In this study, the rice MGDG synthase gene, OsMGD3 was identified and functionally characterized. We showed that plant phosphate (Pi) status and transcription factor OsPHR2 are involved in the transcriptional regulation of OsMGD3. CRISPR/Cas9 knockout (KO) and overexpression (OE) lines of OsMGD3 were generated to explore its potential role in rice adaptation to Pi deficiency. Compared to WT, OsMGD3 KO lines displayed a reduction while OE lines showed an enhanced, Pi acquisition and utilization. Further, OsMGD3 showed a predominant role in roots, altering lateral root growth. Our comprehensive lipidomic analysis revealed the role of OsMGD3 in membrane lipid remodeling in addition to a role in regulating diacylglycerol and phosphatidic acid levels that affected the expression of Pi transporters. Our study highlights the role of OsMGD3 in affecting both internal P utilization and P acquisition in rice.
  • No Thumbnail Available
    Item
    GC-MS-based analysis of methanol: chloroform-extracted fatty acids from plant tissues
    (Bio-protocol LLC., 2018) Patel, Manish Kumar; Das, Shubhashis; Thakur, Jitendra K.
    Fatty acids (FAs) are carboxylic acids with long aliphatic chains that may be straight, branched and saturated or unsaturated. Most of the naturally occurring plant FAs contains an even number of carbon (C4-C24). FAs are used in food and pharmacological industries due to their nutritional importance. In addition, FAs are considered as a promising alternative for the production of biodiesel from terrestrial plant biomass. To establish commercial applications, more reliable analytical methods are needed for the identification, quantification, and composition determination of FAs. Here, we describe a relatively rapid and sensitive method for the extraction, identification, and quantification of FAs from a small quantity of plant tissue. The method includes steps of lipid extraction, conversion of lipid to fatty acid methyl esters (FAMEs) by transmethylation, identification and quantification of FAMEs using gas chromatography-mass spectrometry (GC-MS). In this protocol, an internal standard is added prior to GC-MS analysis. The amount of each FA is calculated from its peak area relative to the peak area of the internal standard.