Institutional Publications

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    Variety-specific transcript accumulation during reproductive stage in drought- stressed rice
    (John Wiley & Sons, 2022) Gour, Pratibha; Kansal, Shivani; Agarwal, Priyanka; Mishra, Bhuwaneshwar Sharan; Sharma, Deepika; Mathur, Saloni; Raghuvanshi, Saurabh
    The divergence of natural stress tolerance mechanisms between species is an intriguing phenomenon. To study it in rice, a comparative transcriptome analysis was carried out in ‘heading’ stage tissue (flag leaf, panicles and roots) of Nagina 22 (N22; drought-tolerant) and IR64 (drought-sensitive) plants subjected to field drought. Interestingly, N22 showed almost double the number of differentially expressed genes (DEGs) than IR64. Many DEGs colocalized within drought-related QTLs responsible for grain yield and drought tolerance and also associated with drought tolerance and critical drought-related plant traits such as leaf rolling, trehalose content, sucrose and cellulose content. Besides, co-expression analysis of the DEGs revealed several ‘hub’ genes known to actively regulate drought stress response. Strikingly, 1366 DEGs, including 21 ‘hub’ genes, showed a distinct opposite regulation in the two rice varieties under similar drought conditions. Annotation of these variety-specific DEGs (VS-DEGs) revealed that they are distributed in various biological pathways. Furthermore, 103 VS-DEGs were found to physically interact with over 1300 genes, including 32 that physically interact with other VS-DEGs as well. The promoter region of these genes have sequence variations among the two rice varieties, which might be in part responsible for their unique expression pattern.
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    Biotechnological interventions for production of therapeutic secondary metabolites using hairy root cultures of medicinal plants
    (Elsevier B.V., 2016) Srivastava, V.; Mehrotra, S.; Verma, Praveen K.
    The repository of molecules (secondary metabolites) in plants have been identified as an effective candidate to cure human diseases. This immense potential is continuously being utilized by exploiting natural plant resources. Such practices led to environmental imbalances and many natural sites were deserted. This chapter provides current perspectives on plant hairy root cultures as an important model for sustainable therapeutic secondary metabolite production. Here, the imperative interventions of biotechnology to enhance the accumulation of such molecules are discussed. Current research trends for metabolite enhancement through media optimization, elicitation, precursor feeding, metabolic engineering, and upscaling are taken into consideration.