Institutional Publications

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Author: search.filters.author.Verma, DeepanjaliSubject: search.filters.subject.MYC2

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    MKK3-MPK6-MYC2 module positively regulates ABA biosynthesis and signalling in Arabidopsis
    (Springer Nature Publishing AG, 2020) Verma, Deepanjali; Bhagat, Prakash Kumar; Sinha, Alok Krishna
    Involvement of MAPK cascades is well studied in the regulation of ABA mediated responses such as stomatal opening, seed germination and stress tolerance. However most of the studies have focused on ABA signalling and involvement of MAPKs in ABA biosynthesis remains elusive. Hence, in the current study, we have identified the new role of MKK3- MPK6-MYC2 module in positive regulation of ABA biosynthesis as well as ABA responsive genes. Seed germination studies disclosed the ABA insensitive phenotype of myc2, mpk6 and mkk3 mutants. Reduced expression of ABA biosynthesis genes, ABA2 and NCED3 in myc2 and MAPK cascade mutants was confirmed by witnessing the direct binding of MYC2 to the promoter of ABA2 in EMSA assays. Additionally, ABA responsive genes also displayed altered expression patterns in these mutants. Further, transcript and biochemical studies validated the role of MPK6 upstream to MYC2 and downstream to MKK3 in response to ABA. Altogether, we have shown that MKK3-MPK6-MYC2 module induces the expression of several ABA related genes and regulate ABA mediated inhibition of seed germination.
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    A bHLH transcription factor, MYC2, imparts salt intolerance by regulating proline biosynthesis in Arabidopsis
    (John Wiley & Sons, 2020) Verma, Deepanjali; Jalmi, Siddhi Kashinath; Bhagat, Prakash Kumar; Verma, Neetu; Sinha, Alok Krishna
    MYC2, a bHLH TF, acts as regulatory hub within several signaling pathways by integration of various endogenous and exogenous signals which shape plant growth and development. However, its involvement in salt stress regulation is still elusive. This study has deciphered a novel role of MYC2 in imparting salt stress intolerance by regulating P5CS1 gene and hence proline synthesis. P5CS1 is a rate limiting enzyme in the biosynthesis of proline. Y‐1‐H and EMSA studies confirmed the binding of MYC2 with the 5’ UTR region of P5CS1. Transcript and biochemical studies have revealed MYC2 as a negative regulator of proline biosynthesis. Proline is necessary for imparting tolerance towards abiotic stress however, its overaccumulation is toxic for the plants. Hence studying the regulation of proline biosynthesis is requisite to understand the mechanism of stress tolerance. We have also studied that MYC2 is regulated by MAPK cascade MKK3‐MPK6 and vice versa. Altogether, this study demonstrates salt stress mediated activation of MYC2 by MAPK cascade, regulating proline biosynthesis and thus salt stress.