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Author: search.filters.author.Ramesh, PalakurthiHas files: Yes

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    Deciphering cold stress resilience: multiomics insights in contrasting wheat genotypes from the western himalayas
    (John Wiley & Sons, 2026) Jan, Sofora; Jan, Farkhandah; Rathore, Mukesh; Singh, Yogita; Kapoor, Prexha; Chaturvedi, Palak; Ghatak, Arindam; Ramesh, Palakurthi; Kumar, Upendra; Prasad, Manoj; Kumar, Sundeep; Rustgi, Sachin; Weckwerth, Wolfram; Kalia, Sanjay; Varshney, Rajeev Kumar; Mir, Reyazul Rouf
    Cold stress threatens wheat productivity, particularly in regions with extreme climatic conditions. To elucidate the molecular mechanisms underlying wheat's response to cold stress, we performed a multiomics analysis integrating lipidomics, transcriptomics, proteomics and metabolomics. Our study focused on two wheat genotypes with contrasting cold tolerance levels, SKAU_52 (tolerant) and SKAU_4301 (susceptible) to capture genotype-specific responses under cold stress. Lipidomic analysis revealed significant changes in lipid composition, with unsaturated lipids such as digalactosyldiacyl glycerols (DGDGs) and monogalactosyldiacylglycerols (MGDGs) upregulated in response to cold stress. These lipids are associated with maintaining membrane fluidity, whereas saturated lipids were downregulated in the cold-tolerant genotype. Transcriptomics analysis provides a strong evidence that cold tolerance in wheat is governed by coordinated activation of the ICE-CBF-COR regulatory cascade, with the cold-tolerant genotype ‘SKAU_52’ showing stronger and more sustained induction across pathway tiers than the cold susceptible wheat genotype ‘SKAU_4301’. Similarly, proteomic data highlighted differential abundance of proteins involved in antioxidative defence, osmotic adjustment and signal transduction, including late embryogenesis abundant (LEA) proteins. Metabolome assessment revealed substantial alterations in carbohydrate and amino acid metabolism, with sucrose and amino acids such as hydroxyproline identified as key contributors to cold tolerance. Additionally, defence hormones such as salicylic acid (SA), jasmonic acid (JA) and abscisic acid (ABA) exhibited genotype-specific regulation with higher accumulation in cold-tolerant genotype. Overall, this integrated multi-omics approach provides novel insights into the complex molecular mechanisms underlying cold stress adaptation in wheat, supporting the development of resilient wheat varieties capable of thriving in challenging cold environments.
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    Targeted lipidome analysis reveals nutritionally enhanced foxtail millet genotypes across diverse grain colours
    (Springer Nature Publishing AG, 2026) Ramesh, Palakurthi; Seni, Sushmita; Singh, Roshan Kumar; Pandey, Ashutosh; Prasad, Manoj
    Foxtail millet (Setaria italica), a small-grained cereal crop, is a rich source of carbohydrates, proteins, minerals, fibers, and lipids, with lipid content ranging from 1–5% of the total grain composition. Whole grain is an excellent natural source of nutraceutical properties and health-beneficial components that significantly reduce chronic inflammation, cardiovascular disease, metabolic syndrome, and type 2 diabetes. Different grain colours in foxtail millet are associated with distinct metabolome composition. However, the relationship between lipid composition and grain colour remains largely unexplored. In this study, a comprehensive metabolomic analysis of eight differently coloured foxtail millet grains led to the identification of 352 distinct metabolites. Among these, 44 metabolites were chemically classified into categories such as fatty acids, steroids, hydrocarbons, benzenes, monoradylglycerols, quinones, and hydroquinones. Linoleic acid was identified as the predominant fatty acid, while lutein emerged as the most abundant carotenoid across all accessions. Gene expression profiling of carotenoid biosynthesis genes revealed significant genotype-specific variations, with SiPSY1, SiPSY2, SiPSY3, SiZDS, SiLCYB, and SiLCYE exhibiting markedly higher expression in the golden yellow genotype SI 101. Furthermore, several unique compounds, including decane 1-iodo, dodecane 4, 6-dimethyl, hexadecane, heptadecane, eicosane, heneicosane, bis (2-ethylhexyl) phthalate, dotriacontane, 2-methylhexacosane, hexatriacontane, squalene, tetrapentacontane, and tetracosane, were identified in foxtail millet grains. These findings provide valuable insights into the metabolic diversity and the differential accumulation of bioactive compounds in among foxtail millet grains with different colours. The study also assists in selecting foxtail millet genotypes with desirable lipid traits for sustainable crop improvement.
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    5M approach to decipher starch-lipid interaction in minor millets
    (Springer Nature Publishing AG, 2023) Ramesh, Palakurthi; Singh, Roshan Kumar; Panchal, Anurag; Prasad, Manoj
    The 5M approach can be applied to understand genetic complexity underlying nutritional traits of minor millets. It will help to systematically identify genomic regions/candidate genes imprinting metabolite profiles. Author’s work in this area is supported by research grants from Ministry of Science and Technology, Gov. of India [GrantCRG/2020/000488 and BT/Ag/Network/Wheat/2019–20].