Institutional Publications

Permanent URI for this collectionhttps://ndkr-library.nipgr.ac.in/handle/123456789/11

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Author: search.filters.author.Kumar, KundanSubject: search.filters.subject.Oryza sativa

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    Genome-wide transcriptome modulation in rice transgenic lines expressing engineered mitogen activated protein kinase kinase 6
    (Taylor & Francis Group, 2014) Kumar, Kundan; Sinha, Alok Krishna
    Mitogen activated protein kinase kinase (MAPKK) is the central module of MAPK cascade and also point of signal integration and divergence. To investigate the regulatory role of OsMKK6, the regulon of genes controlled by OsMKK6 was constructed by microarray analysis between constitutively activated overexpressing transgenic lines and the wild type rice. Regulated genes were identified in overexpressed constitutively activated OsMKK6 and they were further subdivided on the basis of functional categories, viz. transcription, metabolism, signaling, defense and unknown function. These findings suggest the possible physiological role of OsMKK6 in modulating gene expression and signaling pathways during different stresses.
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    Rice WNK1 is regulated by abiotic stress and involved in internal circadian rhythm
    (Landes Bioscience, 2011) Kumar, Kundan; Rao, Kudupudi Prabhakara; Biswas, Dipul Kumar; Sinha, Alok Krishna
    In Mammalian system the WNK (with no lysine kinase) serine-threonine protein kinase gene family is suggested to be involved in regulating ion homeostasis and other pathophysiological processes including cancer, hypertension and renal ion transport. In plant system the information about WNK genes is very poor. However, WNK-like genes have also been identified in plants, including ten in Arabidopsis, designated AtWNK1-AtWNK10. Here we report the cloning and characterization of a homologue of AtWNK1 gene from Oryza sativa indica cultivar Pusa Basmati-1 rice and designated as OsWNK1. The specific feature of this gene is lysine residue in kinase subdomain II, which is essential for the coordination of ATP in the active center and conserved among all other kinases, is absent. OsWNK1 was found to respond differentially under various abiotic stresses like cold, heat, salt, drought. OsWNK1 gene showed rhythmic expression profile under diurnal and circadian conditions at the transcription level. Our data indicates that OsWNK1 in rice might play a role in abiotic stress tolerance and that it is involved in internal rhythm.