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Author: search.filters.author.Kumar, AshutoshSubject: search.filters.subject.Arabidopsis thaliana

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    Role of miRNAs in root development of model plant Arabidopsis thaliana
    (Springer, 2017) Gautam, Vibhav; Singh, Archita; Verma, Swati; Kumar, Ashutosh; Kumar, Pramod; Mahima; Singh, Sharmila; Mishra, Vishnu; Sarkar, Ananda K.
    The molecular regulation of root development is relatively well studied in model plant Arabidopsis as compared to other plants. Besides phytohormones, transcription factors and environmental factors, other important regulators which have recently been shown to play crucial roles in controlling root development are the non-coding RNAs. Small non-coding RNAs of 21–24 nt length (miRNAs and ta-siRNAs) regulate various aspects of plant development by negatively regulating their target genes through transcript cleavage or translational inhibition. In recent past the microRNA-mediated regulation of root development has drawn significant interest in the area of plant research. Several reports have highlighted the role of many miRNAs and ta-siRNAs in root growth, vascular patterning, lateral root (LR) formation and elongation, and adventitious root development, Phytohormones like auxin, cytokinin and environmental factors like light, abiotic and biotic stresses, and nutrient availability influence many miRNA-mediated regulation of root growth and branching. In current review, we summarize the recent advances made in understanding the miRNA-mediated regulation of root development in the model plant Arabidopsis thaliana. The molecular crosstalk between different miRNAs, ta-siRNAs, and concerned target genes that regulate root growth and branching have been addressed.
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    Phytohormonal crosstalk modulates the expression of miR166/165s, target Class III HD-ZIPs, and KANADI genes during root growth in Arabidopsis thaliana
    (Nature Publishing Group, 2017) Singh, Archita; Roy, Shradha; Singh, Sharmila; Das, Shabari Sarkar; Gautam, Vibhav; Yadav, Sandeep; Kumar, Ashutosh; Singh, Alka; Samantha, Sukanya; Sarkar, Ananda K.
    Both phytohormones and non-coding microRNAs (miRNAs) play important role in root development in Arabidopsis thaliana. Mature miR166/165 s, which are derived from precursor transcripts of concerned genes, regulate developmental processes, including leaf and root patterning, by targeting Class III HOMEODOMAIN LEUCINE-ZIPPER (HD-ZIP III) transcription factors (TFs). However, their regulation through hormones remained poorly understood. Here, we show that several phytohormones dynamically regulate the spatio-temporal expression pattern of miR166/165 and target HD-ZIP IIIs in developing roots. Hormone signaling pathway mutants show differential expression pattern of miR166/165, providing further genetic evidence for multilayered regulation of these genes through phytohormones. We further show that a crosstalk of at least six different phytohormones regulate the miR166/165, their target HD-ZIP IIIs, and KANADI (KANs). Our results suggest that HD-ZIP IIIs mediated root development is modulated both transcriptionally through phytohormones and KANs, and post-transcriptionally by miR166/165 that in turn are also regulated by the phytohormonal crosstalk.
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    Apple CALCINEURIN B-LIKE PROTEIN10 genes have evolved to be novel targets of miR167s through sequence variation
    (Current Science Association, 2017) Kumar, Ashutosh; Sarkar, Ananda K.
    The miR167s and its target ARF6/8 are relatively conserved among diverse plant species and have been implicated in reproductive and root development in Arabidopsis. Here we show that some of the CNBL family members have evolved to be targets of miR167s in apple. Despite strong conservation between apple and Arabidopsis CNBLs, AtCNBLs are not miR167 targets. The sequence variation in apple-miR167a and MdCNBLs has created target sites for apple-miR167a in MdCNBL10s. Therefore, we suggest that during the course of evolution, natural selection through sequence variation played a crucial role by choosing different targets among plant species for the same miRNA.