Institutional Publications

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Author: search.filters.author.Jha, GopaljeeHas files: No

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    Detection of endophytic association between Aeschynomene nodulating Bradyrhizobium sp. and traditional Desariya rice roots under rice-Aeschynomene ecosystem of chaur land, Bihar, India
    (Springer Nature Publishing AG, 2022) Rai, Abhilasha; Jha, Manindra Nath; Singh, Devendra; Thapa, Shobit; Chaurasia, Sanjeet Kumar; Jha, Gopaljee
    Engineering diazotrophic rice having either an integral component of diazotrophic microbes or placing microbial origin nif gene to the rice plant is the dream of biotechnologist. Rice-Aeschynomene ecosystem of pristine chaur land provides a suitable niche to search Rhizobium endophytes in rice. Accordingly, the work was initiated to search suitable endophytic Rhizobium strain for artificial symbiosis within the roots of Desariya rice and its source through morphological, biochemical and molecular approaches. Detection of Acetylene reduction assay (ARA) activity in sterilized Desariya rice root confirmed the presence of putative diazotrophic endophytes in rice root. Isolates from Aeschynomene aspera L. nodulating and Desariya rice endophytic Rhizobium were evaluated for growth, IAA, morphological and biochemical features. Carbon profiling pattern of both these isolates indicated that Desariya rice endophytic Rhizobium has its similarity with Aeschynomene aspera L. nodulating Rhizobium. 16S rRNA gene sequencing confirmed the presence of endophytic Bradyrhizobium sp. in Desariya rice roots and its similarity with Aeschynomene aspera L. nodulating Bradyrhizobium. Desariya rice Bradyrhizobium may be an ideal candidate in the future for creating artificial symbiosis in rice due to its similarity with Aeschynomene aspera L. Bradyrhizobium.
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    Understanding the molecular intricacies of rice-Rhizoctonia solani interactions
    (American Phytopathological Society, 2019) Ghosh, S.; Kanwar, P.; Jha, Gopaljee
    Sheath blight disease is a devastating disease in rice that causes huge crop losses worldwide. It is caused by a necrotrophic fungus Rhizoctonia solani. In spite of global efforts till now there are no reports of complete resistance against this pathogen. We observed that R. solani has a brief biotrophic phase wherein mycelial grows parallel to rice veins without showing any morphological and anatomical changes. However, at later stage, i.e. necrotrophic phase, infection cushions along with anatomical changes are observed at the site of disease symptoms. Through transcriptome and metabolome studies we have identified several candidate host susceptibility factors and pathogenicity determinants that might play an important role during pathogenesis of R. solani in rice. Furthermore, using whole genome studies, we have identified several gene/gene families that might contribute to the aggressiveness of the R. solani strains. Although gene manipulation studies in R. solani is difficult we have devised ways wherein we can functionally characterize these pathogenicity determinants. Overall the present study will help in better understanding of the rice-R. solani pathosystem which would eventually be helpful in developing strategies for durable sheath blight disease tolerance in rice.
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    Burkholderia gladioli strain NGJ1 deploys a prophage tail-like protein to feed on fungi
    (American Phytopathological Society, 2019) Swain, D.M.; Yadav, S.; Tyagi, I.; Kumar, R.; Kumar, R.; Ghosh, S.; Das, J.; Jha, Gopaljee
    Fungal pathogens are responsible for approximately two third of the infectious plant diseases. Due to extensive usages, pathogens have evolved resistance against most of the commonly used fungicides. Rice sheath blight disease caused by Rhizoctonia solani remains a serious threat to global sustainable agriculture. The pathogen has a complex biology and has been reported occurring world-wide causing necrosis and dampingoff on numerous host plant species. The pathogen is polymorphic and despite extensive efforts, till today no source of complete disease resistance has been identified against this pathogen. We have recently discovered a novel bacterium Burkholderia gladioli strain NGJ1 which has broad spectrum mycophagus ability. We also demonstrated that the bacteria NGJ1 deploys a prophage tail like protein (Bg_9562) to feed over fungi in a T3SS dependant manner. Moreover we observed that Bg_9562 protein has a broad spectrum antifungal activity on Rhizoctonia solani as well as several other phytopathogenic fungi. Furthermore, being equipped to kill and feed upon fungi, the mycophagous bacteria can serve as an experimental tool box to facilitate discovery of novel anti-fungal molecules. This opens up a new biotechnological application of this prophage tail like protein in controlling fungal diseases in rice as well as in other plants.