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Author: search.filters.author.Deepika, DeepikaSubject: search.filters.subject.Expression

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    Genomic, structural, and molecular analysis of calmodulin-binding transcriptional activators (CAMTAs) suggests their role in plant development and abiotic stress tolerance in chickpea
    (Elsevier B.V., 2025) Sonkar, Kamankshi; Kamali, Saravanappriyan; Kumar, Atul; Deepika, Deepika; Ankit, Ankit; Singh, Amarjeet
    The calmodulin-binding transcriptional activator (CAMTA) transcription factors regulate the expression of target genes in Ca2 + dependent cellular functions. CAMTAs are known to regulate biotic and abiotic stress tolerance, and development in plants. CAMTA family has been characterized in Arabidopsis, it is yet to be explored in the legume plant chickpea. Here, we have identified and characterized the chickpea CAMTA family. Total seven CAMTA genes (CaCAMTA1–7) were identified in chickpea. Gene and domain structure analyses suggested that CAMTAs are structurally conserved. The phylogenetic analysis demarcated CaCAMTAs into three groups namely; group I, II and III, and indicated that CaCAMTAs have co-evolved in dicot leguminous plants whereas, they have divergent evolution in monocots. Protein homology modeling revealed their three-dimensional structure, and composition & conformations of α-helix, β-sheets and p-loops. Subcellular localization showed that CaCAMTA4 was localized both, in the nucleus and the cytosol whereas, CaCAMTA5 was localized in the nucleus. CaCAMTA promoters contain various cis-regulatory elements related to abiotic stresses and plant development. Expression profiling using RNA-seq data revealed differential expression of CaCAMTAs during various stages of plant development. RT-qPCR expression analysis showed that most CaCAMTA genes are drought, salt, and ABA responsive, suggesting their role in abiotic stress tolerance in chickpea. Moreover, CaCAMTA regulon was identified based on the presence of CAMTA binding motif (CGCG box) in the promoters of target genes, and in-silico interaction analysis of TF and putative targets. Overall, CaCAMTAs are crucial for abiotic stress tolerance and plant development in chickpea. Key CaCAMTA genes will be functionally characterized, and will be exploited for developing stress tolerant chickpea varieties.
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    Molecular analysis indicates the involvement of Jasmonic acid biosynthesis pathway in low-potassium (K+) stress response and development in chickpea (Cicer arietinum)
    (Elsevier B.V., 2022) Deepika, Deepika; Ankit; Jonwal, Sarvesh; Mali, Komal Vitthalrao; Sinha, Alok Krishna; Singh, Amarjeet
    K+ is a major macronutrient and its deficiency hampers plant growth and yield. Plants combat low-K+ stress by modifying their root system architecture (RSA). Here, morphophysiological analysis revealed that chickpea plants exhibit sensitivity to low-K+ stress as shown by impaired primary root growth. Phytohormone JA regulates various facets of plant root growth, however, information of JA biosynthesis genes in chickpea is missing. We performed genome-wide identification and molecular characterization of JA biosynthesis pathway genes in chickpea. Total 33 genes belonging to different families i.e., LOXs-18, AOSs-3, AOCs-2, OPRs-6 and JARs-4 were identified in the chickpea genome. In-planta analysis revealed the localization of CaLOX7, − 10, CaAOS1, − 2 and CaAOC1 at subcellular compartments, such as membrane, chloroplast and cytoplasm. Protein expression and in-vitro enzymatic activity analysis showed that CaAOS1 an CaOPR2 are the functional enzymes in chickpea. Promoters of most genes harboured abiotic stress, hormone and development related cis-regulatory elements, suggesting their role in nutrient deficiency, abiotic stress and plant development. qRT-PCR expression profiling showed that about 15 JA biosynthesis genes from different families express differentially whereas, JA catabolism genes were repressed in chickpea root and shoot under low-K+ stress. In addition, JA biosynthesis genes showed differential expression in vegetative and reproductive development, senescence stages, desiccation, salinity and cold stress. These findings indicate the involvement of JA biosynthesis pathway in low-K+ stress response and development in chickpea. Low-K+ stress and development related genes identified in this study could be utilized in genetic engineering of chickpea plants for improved traits.