Institutional Publications

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Author: search.filters.author.Datta, AsisHas files: No

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    Ethylene-mediated regulation of a ripening-specific N-glycan-processing enzyme β-D-N-Acetylhexosaminidase
    (Springer Nature Publishing AG, 2025) Irfan, Mohammad; Kumar, Pankaj; Datta, Asis
    N-glycoproteins in plants play a critical role in various biological processes such as plant stress response, seed development, and fruit ripening. Further, N-glycans and N-glycan-processing enzymes have been identified as critical regulators of fruit ripening in various fruit crop including tomato. Fruit ripening, a multifaceted process influenced by diverse factors is controlled by the upregulation of cell wall degradation enzymes. Among these, N-glycan-processing enzymes, specifically β-D-N-acetylhexosaminidase (β-Hex), are involved in ripening-associated fruit softening, and its suppression enhances fruit shelf life and firmness in both climacteric and non-climacteric fruits. Ethylene is among the various factors that regulates the ripening-specific expression of β-Hex. Notably, ethylene response factor 6 (SlERF6), also known as SlERF.E4, has been identified as a potential transcriptional regulator of β-Hex expression. However, in order to get deeper insights, a thorough understanding of the specific ethylene-mediated control of β-Hex during ripening is crucial. Thus, we present a protocol for investigating the ethylene-mediated regulation of this crucial ripening-related enzyme β-D-N-acetylhexosaminidase.
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    Recent insights into plant circadian clock response against abiotic stress
    (Springer Nature Publishing AG, 2022) Sharma, Megha; Irfan, Mohammad; Kumar, Arun; Kumar, Pankaj; Datta, Asis
    The circadian clock is a cell autonomously and endogenously regulated biological timekeeper that detects changes in environmental stimuli and generates 24-h rhythms that are synched with day to day and periodic oscillations to govern many biological functions. Plant's circadian clocks enable them to anticipate environmental changes by modifying their physiological and biological traits to improve plant fitness. The internal circadian clock not only aids fitness but also allows the plant to time-gate the response to environmental stimuli. The latest evidence on the circadian clock suggests that the clock regulates/modulates the expression of abiotic stress-responsive pathways to improve tolerance to stresses without hampering plant growth. In turn, stress signaling also influences the activity of several clock components. This review emphasizes the interplay of the biological circadian clock with abiotic stress-responsive pathways (drought, heat, cold, and salt) for plant growth and survival as well as for stress resilience. A better comprehension of these mechanisms could aid in the development of genetic tools to improve breeding procedures and plant stress tolerance, thereby increasing crop yield and quality under changing ecological conditions.
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    Application of RNA silencing in improving plant traits for industrial use
    (CABI, 2017) Ghosh, Sumit; Irfan, Mohammad; Datta, Asis
    The discovery of RNA silencing mechanisms has transformed research into gene function and has also allowed novel traits to be developed in plants, including some for industrial applications. To silence the expression of an undesirable gene, RNA silencing may be preferable over direct gene mutation as it leads to a dominant trait. This helps in straightforward screening of the desirable phenotypes. Moreover, RNA silencing is useful in tissue, organ and time-specific silencing of the genes that may play essential roles in plant life and, therefore, direct gene mutation may not be effective. This chapter summarizes the implication of RNA silencing in improving plant traits for industrial applications. Plants developed after RNA silencing of genes that act on cellular organization or metabolism are emerging as convenient and renewable raw materials for industries producing fuels, oils, pharmaceuticals, fibres, papers, seeds and processed foods. Several genetically modified plants, which may not have use in human consumption, have nevertheless been developed for specific industrial applications and await commercial-scale cultivation. Many more industrial crops are under different stages of development by industry and research organizations.