Institutional Publications

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Author: search.filters.author.Chandan, Ravindra K.Has files: Yes

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    The host and pathogen myo-inositol-1-phosphate synthases are required for Rhizoctonia solani AG1-IA infection in tomato
    (John Wiley & Sons, 2024) Tyagi, Kriti; Chandan, Ravindra K.; Sahoo, Debashis; Ghosh, Srayan; Gupta, Santosh Kumar; Jha, Gopaljee
    The myo-inositol-1-phosphate synthase (MIPS) catalyses the biosynthesis of myo-inositol, an important sugar that regulates various physiological and biochemical processes in plants. Here, we provide evidence that host (SlMIPS1) and pathogen (Rs_MIPS) myo-inositol-1-phosphate synthase (MIPS) genes are required for successful infection of Rhizoctonia solani, a devastating necrotrophic fungal pathogen, in tomato. Silencing of either SlMIPS1 or Rs_MIPS prevented disease, whereas an exogenous spray of myo-inositol enhanced disease severity. SlMIPS1 was upregulated upon R. solani infection, and potentially promoted source-to-sink transition, induced SWEET gene expression, and facilitated sugar availability in the infected tissues. In addition, salicylic acid (SA)-jasmonic acid homeostasis was altered and SA-mediated defence was suppressed; therefore, disease was promoted. On the other hand, silencing of SlMIPS1 limited sugar availability and induced SA-mediated defence to prevent R. solani infection. Virus-induced gene silencing of NPR1, a key gene in SA signalling, rendered SlMIPS1-silenced tomato lines susceptible to infection. These analyses suggest that induction of SA-mediated defence imparts disease tolerance in SlMIPS1-silenced tomato lines. In addition, we present evidence that SlMIPS1 and SA negatively regulate each other to modulate the defence response. SA treatment reduced SlMIPS1 expression and myo-inositol content in tomato, whereas myo-inositol treatment prevented SA-mediated defence. We emphasize that downregulation of host/pathogen MIPS can be an important strategy for controlling diseases caused by R. solani in agriculturally important crops.
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    Silencing of tomato CTR1 provides enhanced tolerance against Tomato leaf curl virus infection
    (Taylor & Francis Group, 2019) Chandan, Ravindra K.; Singh, Achuit K.; Patel, Sunita; Swain, Durga Madhab; Tuteja, Narendra; Jha, Gopaljee
    Tomato leaf curl virus(ToLCV) belonging to Begomovirus family of Geminivirus is known to cause one of the most destructive diseases in tomato. Amongst variousToLCVs, a monopartite Tomato leaf curl Joydebpur virus (ToLCJoV) is most prevalent in eastern part of India. In the present study, we observed induced expression of one of the negative regulators of ethylene signaling pathway gene (LeCTR1)in ToLCJoVinfected plants. TheTobacco rattle virus(TRV) induced silencing of theLeCTR1gene provided enhanced tolerance toToLCJoVinfections. The leaf curling as well as ROS accumulation was significantly reduced in the viral infectedLeCTR1silenced plants. Induction of several defense marker genes (NPR1, PR1, PR5, AOS2, EIN2, EIN3and ERF5) reinforced enhanced tolerance againstToLCJoVinfection in the LeCTR1silenced tomato. Overall, the present study provides evidence that silencing ofLeCTR1can be deployed to protect tomato fromToLCJoVinfections.