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Author: search.filters.author.Chakraborty, NiranjanSubject: search.filters.subject.Plant immunity

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    Systemic acquired resistance specifc proteome of Arabidopsis thaliana
    (Springer Nature Publishing AG, 2020) Kumar, Rajiv; Barua, Pragya; Chakraborty, Niranjan; Nandi, Ashis Kumar
    Key message A comparative proteomic study between WT and SAR-compromised rsi1/fd mutant reveals a set of proteins having possible roles in the SAR development. Abstract A partly infected plant shows enhanced resistance during subsequent infection through the development of systemic acquired resistance (SAR). Mobile signals generated at the site of primary infection travel across the plant for the activation of SAR. These mobile signals are likely to cause changes in the expression of a set of proteins in the distal tissue, which contributes to the SAR development. However, SAR-specifc proteome is not revealed for any plant. The reduced systemic immunity 1 (rsi1)/(allelic to fowering locus D; fd) mutant of Arabidopsis is compromised for SAR but shows normal local resistance. Here we report the SAR-specifc proteome of Arabidopsis by comparing diferentially abundant proteins (DAPs) between WT and fd mutant. Plants were either mock-treated or SAR-induced by primary pathogen inoculation. For proteomic analysis, samples were collected from the systemic tissues before and after the secondary inoculation. Protein identifcation was carried out by using two-dimensional gel electrophoresis (2-DE) followed by tandem mass spectrometry. Our work identifed a total of 94 DAPs between mock and pathogen treatment in WT and fd mutant. The DAPs were categorized into diferent functional groups along with their subcellular localization. The majority of DAPs are involved in metabolic processes and stress response. Among the subcellular compartments, plastids contained the highest number of DAPs, suggesting the importance of plastidic proteins in SAR activation. The fndings of this study would provide resources to engineer efcient SAR activation traits in Arabidopsis and other plants.
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    Integrative network analyses of wilt transcriptome in chickpea reveal genotype dependent regulatory hubs in immunity and susceptibility
    (Springer Nature, 2018) Ashraf, Nasheeman; Basu, Swaraj; Narula, Kanika; Ghosh, Sudip; Tayal, Rajul; Gangisetty, Nagaraju; Biswas, Sushmita; Aggarwal, Pooja R.; Chakraborty, Niranjan; Chakraborty, Subhra
    Host specific resistance and non-host resistance are two plant immune responses to counter pathogen invasion. Gene network organizing principles leading to quantitative differences in resistant and susceptible host during host specific resistance are poorly understood. Vascular wilt caused by root pathogen Fusarium species is complex and governed by host specific resistance in crop plants, including chickpea. Here, we temporally profiled two contrasting chickpea genotypes in disease and immune state to better understand gene expression switches in host specific resistance. Integrative gene-regulatory network elucidated tangible insight into interaction coordinators leading to pathway determination governing distinct (disease or immune) phenotypes. Global network analysis identified five major hubs with 389 co-regulated genes. Functional enrichment revealed immunome containing three subnetworks involving CTI, PTI and ETI and wilt diseasome encompassing four subnetworks highlighting pathogen perception, penetration, colonization and disease establishment. These subnetworks likely represent key components that coordinate various biological processes favouring defence or disease. Furthermore, we identified core 76 disease/immunity related genes through subcellular analysis. Our regularized network with robust statistical assessment captured known and unexpected gene interaction, candidate novel regulators as future biomarkers and first time showed system-wide quantitative architecture corresponding to genotypic characteristics in wilt landscape.