Institutional Publications

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Author: search.filters.author.Bhadouria, JyotiSubject: search.filters.subject.PUE

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    Monogalactosyl Diacylglycerol Synthase 3 affects phosphate utilization and acquisition in rice
    (Oxford University Press, 2022) Verma, Lokesh; Bhadouria, Jyoti; Rupam, Bhunia Kumar; Singh, Shweta; Panchal, Poonam; Bhatia, Chitra; Eastmond, Peter J; Giri, Jitender
    Galactolipids are essential for compensating for the loss of phospholipids by “membrane lipid remodeling” in plants under phosphorus (P) deficiency. Monogalactosyl diacylglycerol (MGDG) synthases catalyze the synthesis of MGDG which is further converted into digalactosyl diacylglycerol (DGDG), later replacing phospholipids in the extraplastidial membranes. However, the roles of these enzymes are not well explored in rice. In this study, the rice MGDG synthase gene, OsMGD3 was identified and functionally characterized. We showed that plant phosphate (Pi) status and transcription factor OsPHR2 are involved in the transcriptional regulation of OsMGD3. CRISPR/Cas9 knockout (KO) and overexpression (OE) lines of OsMGD3 were generated to explore its potential role in rice adaptation to Pi deficiency. Compared to WT, OsMGD3 KO lines displayed a reduction while OE lines showed an enhanced, Pi acquisition and utilization. Further, OsMGD3 showed a predominant role in roots, altering lateral root growth. Our comprehensive lipidomic analysis revealed the role of OsMGD3 in membrane lipid remodeling in addition to a role in regulating diacylglycerol and phosphatidic acid levels that affected the expression of Pi transporters. Our study highlights the role of OsMGD3 in affecting both internal P utilization and P acquisition in rice.
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    Purple acid phosphatases: roles in phosphate utilization and new emerging functions
    (Springer Nature Publishing AG, 2022) Bhadouria, Jyoti; Giri, Jitender
    Plants strive for phosphorus (P), which is an essential mineral for their life. Since P availability is limiting in most of the world’s soils, plants have evolved with a complex network of genes and their regulatory mechanisms to cope with soil P defciency. Among them, purple acid phosphatases (PAPs) are predominantly associated with P remobilization within the plant and acquisition from the soil by hydrolyzing organic P compounds. P in such compounds remains otherwise unavailable to plants for assimilation. PAPs are ubiquitous in plants, and similar enzymes exist in bacteria, fungi, mammals, and unicellular eukaryotes, but having some diferences in their catalytic center. In the recent past, PAPs’ roles have been extended to multiple plant processes like fowering, seed development, senescence, carbon metabolism, response to biotic and abiotic stresses, signaling, and root development. While new functions have been assigned to PAPs, the underlying mechanisms remained understood poorly. Here, we review the known functions of PAPs, the regulatory mechanisms, and their relevance in crop improvement for P-use-efciency. We then discuss the mechanisms behind their functions and propose areas worthy of future research. Finally, we argue that PAPs could be a potential target for improving P utilization in crops. In turn, this is essential for sustainable agriculture.