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    Quantification of pollen viability in Lantana camara by digital holographic microscopy
    (Cambridge University Press, 2023) Kumar, Vipin; Goyal, Nishant; Prasad, Abhishek; Babu, Suresh; Khare, Kedar; Yadav, Gitanjali
    Pollen grains represent the male gametes of seed plants and their viability is critical for sexual reproduction in the plant life cycle. Palynology and viability studies have traditionally been used to address a range of botanical, ecological and geological questions, but recent work has revealed the importance of pollen viability in invasion biology as well. Here, we report an efficient visual method for assessing the viability of pollen using digital holographic microscopy (DHM). Imaging data reveal that quantitative phase information provided by the technique can be correlated with viability as indicated by the outcome of the colorimetric test. We successfully test this method on pollen grains of Lantana camara, a well-known alien invasive plant in the tropical world. Our results show that pollen viability may be assessed accurately without the usual staining procedure and suggest potential applications of the DHM methodology to a number of emerging areas in plant science.
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    Ecological networks in urban forest fragments reveal species associations between native and invasive plant communities
    (MDPI AG, 2022) Chauhan, Sonali; Yadav, Gitanjali; Babu, Suresh
    Forest fragments are characteristic features of many megacities that have survived the urbanisation process and are often represented by unique assemblages of flora and fauna. Such woodlands are representations of nature in the city—often dominated by non-native and invasive species that coexist with resilient native congeners and purposefully introduced flora. These forest fragments also provide significant ecosystem services to urban society and therefore, understanding their compositional patterns is of considerable importance for conservation and management. In this work, we use a complex network approach to investigate species assemblages across six distinct urban forest fragments in the South Delhi Ridge area of the National Capital Territory, India. We generate bipartite ecological networks using conventional vegetation sampling datasets, followed by network partitioning to identify multiple cliques across the six forest fragments. Our results show that urban woodlands primarily form invasive–native associations, and that major invasive species, such as Prosopis juliflora and Lantana camara exclude each other while forming cliques. Our findings have implications for the conservation of these urban forests and highlight the importance of using network approaches in vegetation analysis.
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    Decision support systems based on scientific evidence: bibliometric networks of invasive Lantana camara
    (Indian National Science Academy, 2021) Mishra, Preet; Prasad, Abhishek; Babu, Suresh; Yadav, Gitanjali
    Extraction and analysis of useful knowledge from the vast amount of relevant published literature can add valuable insights to any research theme or area of interest. We introduce a simplifed bibliometric data analysis protocol for gaining substantial insights into research thematics, which can also serve as a handy practical skill for researchers while working from home. In this paper, we provide ways of developing a holistic research strategy using bibliometric-data driven approaches that integrate network analysis and information management, without the need for full paper access. This protocol is a comprehensive multi-modular pathway for analysis of metadata obtained from major scientifc publishing houses by the use of a Decision Support System (DSS). A simple case study on the invasive species Lantana camara has been presented as a proof-of-concept to show how one can implement this DSS based protocol. Some perspectives are also provided on how the outcomes can be used directly or scaled up for long term research interventions. We hope that this work will simplify exploratory literature review, and enable rational design of research objectives for scholars, as well as the development of comprehensive grant proposals that address gaps in research.