Browsing by Author "Yang, Jing"

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    Rice actin binding protein RMD controls crown root angle in response to external phosphate
    (Springer Nature, 2018) Huang, Guoqiang; Liang, Wanqi; Sturrock, Craig J.; Pandey, Bipin K.; Giri, Jitender; Mairhofer, Stefan; Wang, Daoyang; Muller, Lukas; Tan, Hexin; York, Larry M.; Yang, Jing; Song, Yu; Kim, Yu-Jin; Qiao, Yang; Xu, Jian; Kepinski, Stefan; Bennett, Malcolm J.; Zhang, Dabing
    Root angle has a major impact on acquisition of nutrients like phosphate that accumulate in topsoil and in many species; low phosphate induces shallower root growth as an adaptive response. Identifying genes and mechanisms controlling root angle is therefore of paramount importance to plant breeding. Here we show that the actin-binding protein Rice Morphology Determinant (RMD) controls root growth angle by linking actin filaments and gravity-sensing organelles termed statoliths. RMD is upregulated in response to low external phosphate and mutants lacking of RMD have steeper crown root growth angles that are unresponsive to phosphate levels. RMD protein localizes to the surface of statoliths, and rmd mutants exhibit faster gravitropic response owing to more rapid statoliths movement. We conclude that adaptive changes to root angle in response to external phosphate availability are RMD dependent, providing a potential target for breeders.
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    Rice auxin influx carrier OsAUX1 facilitates root hair elongation in response to low external phosphate
    (Nature Publishing Group, 2018) Giri, Jitender; Bhosale, Rahul; Huang, Guoqiang; Pandey, Bipin K.; Parker, Helen; Zappala, Susan; Yang, Jing; Dievart, Anne; Bureau, Charlotte; Ljung, Karin; Price, Adam; Rose, Terry; Larrieu, Antoine; Mairhofer, Stefan; Sturrock, Craig J.; White, Philip; Dupuy, Lionel; Hawkesford, Malcolm; Perin, Christophe; Liang, Wanqi; Peret, Benjamin; Hodgman, Charlie T.; Lynch, Jonathan; Wissuwa, Matthias; Zhang, Dabing; Pridmore, Tony; Mooney, Sacha J.; Guiderdoni, Emmanuel; Swarup, Ranjan; Bennett, Malcolm J.
    Root traits such as root angle and hair length influence resource acquisition particularly for immobile nutrients like phosphorus (P). Here, we attempted to modify root angle in rice by disrupting the OsAUX1 auxin influx transporter gene in an effort to improve rice P acquisition efficiency. We show by X-ray microCT imaging that root angle is altered in the osaux1 mutant, causing preferential foraging in the top soil where P normally accumulates, yet surprisingly, P acquisition efficiency does not improve. Through closer investigation, we reveal that OsAUX1 also promotes root hair elongation in response to P limitation. Reporter studies reveal that auxin response increases in the root hair zone in low P environments. We demonstrate that OsAUX1 functions to mobilize auxin from the root apex to the differentiation zone where this signal promotes hair elongation when roots encounter low external P. We conclude that auxin and OsAUX1 play key roles in promoting root foraging for P in rice.