Browsing by Author "Yadav, Reena"

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    Modulation of nitric oxide mediated by Phytoglobin1 plays a role in salinity tolerance via reduced nitro-oxidative stress in Arabidopsis
    (Elsevier B.V., 2026) Swain, Jagannath; Babuta, Priyanka; Pandey, Sonika; Samant, Sanjib Bal; Yadav, Reena; Manbir; Hebelstrup, Kim H.; Igamberdiev, Abir U.; Singla-Pareek, Sneh Lata; Pareek, Ashwani; Gupta, Kapuganti Jagadis
    Salinity is one of the major abiotic stresses that induces nitro-oxidative stress, which severely diminishes plant growth, development, and survival by altering various metabolic pathways. Phytoglobin (Pgb) is a nitric oxide (NO) scavenger that plays an important role in various stresses. However, the role of differential levels of phytoglobin1 in regulation of salinity stress induced nitro-oxidative stress in plants is not known. Here we characterized the role of Pgb-mediated NO in salinity tolerance by regulation of nitro-oxidative stress using Pgb1 overexpressing (Pgb1-OE) and silencing lines (pgb1-AS) of Arabidopsis. We found that imposing salinity leads to enhanced expression of Pgb1. NO measurement by both chemiluminescence and DAF-FM-DA suggested that salinity stress induces NO production. Pgb1-OE lines showed reduced levels of NO which is accompanied by reduced ROS, superoxide and H2O2 levels. On the contrary, pgb1-AS lines showed increased NO and ROS under salt stress. Further, gene expression analysis revealed an elevated expression of antioxidant genes in Pgb1-OE line in comparison to WT and pgb1-AS lines under salinity stress. Pgb1-OE lines showed enhanced survival which is correlated with reduced peroxynitrite and tyrosine nitration and opposing effect was observed in pgb1-AS lines along with increased cell death. Taken together, our study revealed that modulation of Pgb1 enhances tolerance to salinity-induced nitro-oxidative stress.
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    Overexpression of phytoglobin1 in rice leads to enhanced nitrogen use efficiency via modulation of nitric oxide
    (John Wiley & Sons, 2025) Samant, Sanjib Bal; Swain, Jagannath; Yadav, Nidhi; Yadav, Reena; Singh, Pooja; Rai, Preeti; Sheri, Vijay; Sreeman, Sheshshayee; Subramanyam, Rajagopal; Pareek, Ashwani; Gupta, Kapuganti Jagadis
    Nitric oxide (NO) is one of the byproducts of nitrogen metabolism. Excess amount of NO is scavenged by phytoglobins. The role of phytoglobin mediated NO homoeostasis in modulation of nitrate transporters was investigated using NO scavenger cPTIO, phytoglobin overexpressing rice and Arabidopsis. Growing plants under low nitrate leads to generation of reduced levels of NO accompanied by elevated expression of high affinity transporters (HATs) such as NRT2.1, NRT2.3 and NRT2.4. Scavenging of NO by cPTIO under optimal nitrate caused enhanced HATs expression. Phytoglobin overexpressing Arabidopsis showed improved growth and enhanced expression of HATs under low nitrogen in comparison to WT. Pretreatment of optimal nitrate grown plants with NO scavenger cPTIO enhanced HATs expression and shifting of these primed plants from optimal to low nitrate leads to further elevation of HATs expression accompanied by enhanced nitrogen uptake and its accumulation with positive effect on growth. Phytoglobin overexpression in rice leads to enhanced HATs expression, improved growth, nitrogen accumulation under low nitrate. Pgb OE lines showed enhanced accumulation of amino acids. Taken together our results suggest an important role of phytoglobins in nitrogen uptake and assimilation.