Browsing by Author "Upadhyaya, Kailash C."

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    Differential transcript accumulation in chickpea during early phases of compatible interaction with a necrotrophic fungus Ascochyta rabiei
    (Springer Science, 2012) Jaiswal, Purnima; Cheruku, Jyothi Reddy; Kumar, Kamal; Yadav, Saurabh; Singh, Archana; Kumari, Pragati; Dube, Sunil Chandra; Upadhyaya, Kailash C.; Verma, Praveen K.
    The initial phases of the disease establishment are very crucial for the compatible interactions. Pathogens must overcome the responses generated by the host for the onset of disease invasion. The compatible interaction is inadequately represented in plant-pathogen interaction studies. To gain broader insight into the early responses elicited by chickpea blight fungus Ascochyta rabiei during compatible interaction; we isolated early responsive genes of chickpea using PCR based suppression subtractive hybridization (SSH) strategy. We obtained ~250 unique genes after homology search and redundancy elimination. Based on their potential cellular functions, these genes were broadly classified into eleven different categories viz. stress, signaling, gene regulation, cellular metabolism and genes of unknown functions. Present study revealed few unexpected genes which have a possible role in induced immunity and disease progression. We employed macroarray, northern blot, real-time PCR and cluster analysis to develop transcript profiles. Most of the genes analyzed were early induced and were transcriptionally upregulated upon 24 h post inoculation. Our approach has rendered the isolation of early responsive genes involved in signaling and regulation of metabolic changes upon fungal infection. The information obtained will help to dissect the molecular mechanisms during compatible chickpea-Ascochyta interactions.
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    Lectin protein kinase is induced in plant roots in response to the endophytic fungus, Piriformospora indica
    (Springer, 2017) Nivedita; Verma, Praveen K.; Upadhyaya, Kailash C.
    The symbiotic association of Piriformospora indica provides growth promotion leading to increased biomass as well as enhanced plant tolerance to biotic and abiotic stresses in a variety of plant species. Since various protein kinases are thought to be involved in mutual signaling in symbiotic interactions, we analyzed transcript profile of rice protein kinase genes during the infection process by real-time PCR. A rice L-type lectin protein kinase (OslecRK) and Arabidopsis AtLecRK genes are highly upregulated in roots during the initial stage of symbiotic association. In order to investigate the role of LecRK gene in root colonization, the Arabidopsis mutant line (Atlecrk) of rice homolog was used which shows the restricted intracellular penetration of the P. indica hyphae. The results indicate that LecRK might be a crucial factor in perception and recognition during plant–P. indica association by regulating fungal invasion.