Browsing by Author "Tuteja, Narendra"

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Abiotic stress-responsive expression of wali1 and wali5 genes from wheat
(Landes Bioscience, 2012) Garg, Bharti; Puranik, Swati; Tuteja, Narendra; Prasad, Manoj
Two cDNA clones, encoding Aluminum-responsive wali1 and wali5, were identified in dehydration stress-specific cDNA library from wheat. Their sequence variations and structural dissimilarities indicated them to be non-homologous genes. Expression of both genes was induced by various abiotic stresses as well as in response to plant hormones and oxidative molecules. Further, they were expressed differentially in shoot and root tissues of wheat seedlings, their transcripts being specifically abundant in roots. Previously characterized as being only Aluminum treatment induced, this report proposes them as novel candidates for stress-responsive studies.
Concurrent overexpression of rice G-protein β and γ subunits provide enhanced tolerance to sheath blight disease and abiotic stress in rice
(Springer Nature Publishing AG, 2019) Swain, Durga Madhab; Sahoo, Ranjan Kumar; Chandan, Ravindra Kumar; Ghosh, Srayan; Kumar, Rahul; Jha, Gopaljee; Tuteja, Narendra
The heterotrimeric G-proteins act as signalling molecules and modulate various cellular responses including stress tolerance in eukaryotes. The gamma (γ) subunit of rice G-protein (RGG1) was earlier reported to promote salinity stress tolerance in rice. In the present study, we report that a rice gene-encoding beta (β) subunit of G-protein (RGB1) gets upregulated during both biotic (upon a necrotrophic fungal pathogen, Rhizoctonia solani infection) and drought stresses. Markerfree transgenic IR64 rice lines that simultaneously overexpress both RGB1 and RGG1 genes under CaMV35S promoter were raised. The overexpressing (OE) lines showed enhanced tolerance to R. solani infection and salinity/drought stresses. Several defense marker genes including OsMPK3 were signifcantly upregulated in the R. solani-infected OE lines. We also found the antioxidant machineries to be upregulated during salinity as well as drought stress in the OE lines. Overall, the present study provides evidence that concurrent overexpression of G-protein subunits (RGG1 and RGB1) impart multiple (both biotic and abiotic) stress tolerance in rice which could be due to the enhanced expression of stress-marker genes and better management of reactive oxygen species (ROS)-scavenging/photosynthetic machinery. The current study suggests an improved approach for simultaneous improvement of biotic and abiotic stress tolerance in rice which remains a major challenge for its sustainable cultivation.
Heterologous overexpression of PDH45 gene of pea provides tolerance against sheath blight disease and drought stress in rice
(Elsevier B.V., 2022) Sahoo, Ranjan Kumar; Chandan, Ravindra Kumar; Swain, Durga Madhab; Tuteja, Narendra; Jha, Gopaljee
Biotic and abiotic stress tolerant crops are required for sustainable agriculture as well as ensuring global food security. In a previous study, we have reported that heterologous overexpression of pea DNA helicase (PDH45), a DEAD-box family member protein, provides salinity stress tolerance in rice. The improved management of photosynthetic machinery and scavenging of reactive oxygen species (ROS) are associated with PDH45 mediated salinity stress tolerance. However, the role of PDH45 in biotic and other abiotic stress (drought) tolerance remains unexplored. In the present study, we have generated marker-free transgenic IR64 rice lines that overexpress PDH45 under the CaMV35S promoter. The transgenic rice lines exhibited a significant level of tolerance against sheath blight disease, caused by Rhizoctonia solani, a polyphagous necrotrophic fungal pathogen. The defense as well as antioxidant responsive marker genes were significantly upregulated in the PDH45 overexpressing (OE) rice lines, upon pathogen infection. Moreover, the OE lines exhibited tolerance to drought stress and various antioxidant as well as drought responsive marker genes were significantly upregulated in them, upon drought stress. Overall, the current study emphasizes that heterologous overexpression of PDH45 provides abiotic as well as biotic stress tolerance in rice. Tolerance against drought as well as sheath blight disease by overexpression of a single gene (PDH45) signifies the practical implication of the present study. Moreover, considering the conserved nature of the gene in different plant species, we anticipate that PDH45 can be gainfully deployed to impart tolerance against multiple stresses in agriculturally important crops.
Mitogen-activated protein kinase signaling in plant under abiotic stress
(Landes Bioscience, 2011) Sinha, Alok Krishna; Jaggi, Monika; Raghuram, Badmi; Tuteja, Narendra
Mitogen-activated protein kinase cascade is evolutionarily conserved signal transduction module involved in transducing extracellular signals to the nucleus for appropriate cellular adjustment. This cascade consists essentially of three components, a MAPK kinase kinase (MAPKKK), a MAPK kinase (MAPKK) and a MAPK connected to each other by the event of phosphorylation. These kinases play various roles in intra- and extra-cellular signaling in plants by transferring the information from sensors to responses. Signaling through MAP kinase cascade can lead to cellular responses including cell division, differentiation as well as responses to various stresses. MAPK signaling has also been associated with hormonal responses. In plants, MAP kinases are represented by multigene families and are involved in efficient transmission of specific stimuli and also involved in the regulation of the antioxidant defense system in response to stress signaling. In the current review we summarize and investigate the participation of MAPKs as possible mediators of various abiotic stresses in plants.
Mitogen-activated protein kinases in abiotic stress tolerance in crop plants: "-omics" approaches
(Wiley-VCH Verlag GmbH & Co., 2013) Jaggi, Monika; Gupta, Meetu; Tuteja, Narendra; Sinha, Alok Krishna
Plants, in order to grow and survive, need to counter a plethora of stresses, both biotic and abiotic. During the process of evolution, plants have developed sophisticated mechanisms to perceive these stresses and transduce them down to the nucleus for appropriate cellular adjustment. Phosphorylation of proteins is one of the important mechanisms for controlling many fundamental cellular processes in all living organisms. A network of mitogen-activated protein kinases (MAPKs) is an evolutionarily conserved phosphorelay cascade among animals, plants, and yeasts that transduces a variety of signals from cell surfaces to the nucleus. This unique protein cascade is also involved in the development and survival of the plants. This cascade consists essentially of three components, a MAPK kinase kinase (MAPKKK), a MAPK kinase (MAPKK), and a MAPK connected to each other by the event of phosphorylation. Signaling through MAP kinase cascade can lead to cellular responses including cell division and differentiation as well as responses to various stresses. In plants, MAP kinases are represented by multigene families and are involved in efficient transmission of specific stimuli and also involved in the regulation of the antioxidant defense system in response to stress signaling. In this chapter, we summarize and investigate the participation of MAPKs as possible mediators of various abiotic stresses in plants. We also focus on recent progress in integrated transcriptomics, proteomics, and metabolomics analyses of MAPK signaling pathway that regulates plant physiological processes during abiotic stress responses. We also deal with the limitations and future prospects of these “-omics” approaches.
Piriformospora indica: a friend in need is a friend in deed
(Research and Reviews, 2016) Trivedi, Dipesh Kumar; Srivastava, Amit; Verma, Praveen K.; Tuteja, Narendra; Gill, Sarvajeet Singh
Piriformospora indica is an endophytic root-colonizing fungal species classified in the genus Piriformospora of the order Sebacinales. The chlamydospores of P. indica has a typical pear-shape and it was first discovered from the orchid plants in the Thar desert in Rajasthan, India and thus named P. indica.
Silencing of tomato CTR1 provides enhanced tolerance against Tomato leaf curl virus infection
(Taylor & Francis Group, 2019) Chandan, Ravindra K.; Singh, Achuit K.; Patel, Sunita; Swain, Durga Madhab; Tuteja, Narendra; Jha, Gopaljee
Tomato leaf curl virus(ToLCV) belonging to Begomovirus family of Geminivirus is known to cause one of the most destructive diseases in tomato. Amongst variousToLCVs, a monopartite Tomato leaf curl Joydebpur virus (ToLCJoV) is most prevalent in eastern part of India. In the present study, we observed induced expression of one of the negative regulators of ethylene signaling pathway gene (LeCTR1)in ToLCJoVinfected plants. TheTobacco rattle virus(TRV) induced silencing of theLeCTR1gene provided enhanced tolerance toToLCJoVinfections. The leaf curling as well as ROS accumulation was significantly reduced in the viral infectedLeCTR1silenced plants. Induction of several defense marker genes (NPR1, PR1, PR5, AOS2, EIN2, EIN3and ERF5) reinforced enhanced tolerance againstToLCJoVinfection in the LeCTR1silenced tomato. Overall, the present study provides evidence that silencing ofLeCTR1can be deployed to protect tomato fromToLCJoVinfections.
Stress induced beta subunit of heterotrimeric G-proteins from Pisum sativum interacts with mitogen activated protein kinase
(Landes Bioscience, 2011) Bhardwaj, Deepak; Sheikh, Arsheed Hussain; Sinha, Alok Krishna; Tuteja, Narendra
We here report in Pisum sativum system a novel protein-protein interaction of β-subunit of heterotrimeric G-proteins (PsGβ) with a Mitogen activated protein kinase (PsMPK3) during cDNA library screening by yeast-two-hybrid assay. The transcript of these two genes also showed co-regulation under abscisic acid (ABA) and methyl jasmonate (MeJA) treatments. The protein-protein interaction was further validated by performing one-to-one interaction and β-galactosidase assay in yeast system. β-subunit of G-proteins from a heterologous system Oryzae sativa also showed interaction with PsMPK3. The interaction between PsGβ and PsMPK3 was further confirmed by in vitro protein-protein interaction. This suggested that MPK3 function as effector molecule for Gβ, which may helps in the regulation of stomatal functioning. These findings also provide an evidence for a possible cross-talk between MPK3 and G-protein-mediated signaling pathways in plants.