Browsing by Author "Solanke, Amolkumar U."

Filter results by typing the first few letters
Now showing 1 - 2 of 2
  • Thumbnail Image
    Item
    Characterization and phylogenetic analysis of environmental stress responsive SAP gene family encoding A20/AN1 zinc-finger proteins in tomato
    (Springer, 2009) Solanke, Amolkumar U.; Sharma, Manoj K.; Tyagi, Akhilesh K.; Sharma, Arun Kumar
    Characterization of genes responsive to stress is important for eVorts on improving stress tolerance of plants. To address components involved in stress tolerance of tomato (Solanum lycopersicum), a stress-responsive gene family encoding A20/AN1 zinc Wnger proteins was characterized. In the present study, 13 members of this gene family were cloned from tomato cultivar Pusa Ruby and named as Stress Associated Protein (SAP) genes. Out of 13 genes, 12 have been mapped on their respective chromo- somes. Expression of these genes in response to cold, heat, salt, desiccation, wounding, abscisic acid, oxidative and submergence stresses was analysed. All tomato SAP genes were found to be responsive to one or other type of environ- mental stress. The phylogenetic analysis of these genes, along with their orthologs from Solanaceae species sug- gests the presence of a common set of SAP genes in the studied Solanaceae species. The present study characterizes a SAP gene family, which encodes A20/AN1 zinc Wnger containing proteins from tomato for the Wrst time. Genes showing high expression in response to a particular stress can be exploited for improving stress tolerance of tomato and other Solanaceae members.
  • Thumbnail Image
    Item
    Identification, phylogeny and transcript profiling of ERF family genes during development and abiotic stress treatments in tomato
    (Springer, 2010) Sharma, Manoj K.; Kumar, Rahul; Solanke, Amolkumar U.; Sharma, Rita; Tyagi, Akhilesh K.; Sharma, Arun K.
    Ethylene responsive transcription factors have been shown to be intimately connected to plant development, defense responses and stress signaling pathways and in order to use them for plant improvement, we need to have better understanding of these proteins. In this study, 85 ERF genes have been identified from tomato using raw EST data in various public repositories. Phylogenetic analysis with tomato ERF domains revealed their distribution in all the groups, previously identified in model systems. MEME motif analysis resulted in identification of conserved domains, characteristic to member of each clade, in addition to ERF domain. Expression analysis during vegetative and reproductive stages of development using QPCR and tomato GeneChip arrays, revealed their tissue-specific/preferential accumulation. In total, 57 genes were found to be differentially expressed during temporal stages of tomato fruit development. The expression analysis of 23 ERF family genes representing each clade in response to seven abiotic stress treatments revealed their differential expression in response to more than one abiotic stress treatments. Results suggest that ERF genes play diverse roles in plant's life and comprehensive data generated will be helpful in conducting functional genomics studies to understand their precise role during plant development and stress response.