Browsing by Author "Sivalingam, Palaiyur Nanjappan"

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GBF3 transcription factor imparts drought tolerance in Arabidopsis thaliana
(Nature Publishing Group, 2017) Ramegowda, Venkategowda; Gill, Upinder Singh; Sivalingam, Palaiyur Nanjappan; Gupta, Aarti; Gupta, Chirag; Govind, Geetha; Nataraja, Karaba N.; Pereira, Andy; Udayakumar, Makarla; Mysore, Kirankumar S.; Senthil-Kumar, Muthappa
Drought transcriptome analysis of finger millet (Eleusine coracana) by cDNA subtraction identified drought responsive genes that have a potential role in drought tolerance. Through virus-induced gene silencing (VIGS) in a related crop species, maize (Zea mays), several genes, including a G-BOX BINDING FACTOR 3 (GBF3) were identified as candidate drought stress response genes and the role of GBF3 in drought tolerance was studied in Arabidopsis thaliana. Overexpression of both EcGBF3 and AtGBF3 in A. thaliana resulted in improved tolerance to osmotic stress, salinity and drought stress in addition to conferring insensitivity to ABA. Conversely, loss of function of this gene increased the sensitivity of A. thaliana plants to drought stress. EcGBF3 transgenic A. thaliana results also suggest that drought tolerance of sensitive plants can be improved by transferring genes from far related crops like finger millet. Our results demonstrate the role of GBF3 in imparting drought tolerance in A. thaliana and indicate the conserved role of this gene in drought and other abiotic stress tolerance in several plant species.
Non-host resistance to plant viruses: What do we know?
(Elsevier B.V., 2020) Baruah, Aiswarya; Sivalingam, Palaiyur Nanjappan; Fatima, Urooj; Senthil-Kumar, Muthappa
Non-host resistance (NHR) is a prevalent mode of plant defense against invading pathogens, including viruses. Being hard to circumvent, NHR has considerable potential to provide durable resistance against virus infection. This review systematically analyzes recent research findings demonstrating NHR mechanisms and chronologically enumerates the defense strategies contributing to NHR. Conventionally, the NHR mechanisms that operate by restricting viral entry and multiplication are termed “true NHR.” Here, we propose NHR mechanisms operating beyond viral multiplication in non-host plants as “apparent NHR.” This review elucidates both categories of NHR and provides insights into the mechanisms involved in NHR.
Plant responses to concurrent abiotic and biotic stress: unravelling physiological and morphological mechanisms
(Springer Nature Publishing AG, 2024) Dixit, Shikha; Sivalingam, Palaiyur Nanjappan; Baskaran, R. K. Murali; Senthil-Kumar, Muthappa; Ghosh, Probir Kumar
With the increasing impact of climate change and global warming, not only abiotic stress factors have gained prominence, but their infuence on plant–biotic interaction has also increased. Plants respond diferently to abiotic factors compared to pests and pathogens, which thrive under intense climatic conditions, leading to higher disease susceptibility and potential epidemic outbreaks. Therefore, a comprehensive understanding of the efects of concurrent biotic and abiotic stress on plants is essential. Despite its signifcance, there have been limited studies on the physiological and morphological responses of plants to combined stress, and the underlying molecular mechanisms remain elusive. While model crops like rice and maize have been explored under the context to some extent there is a scarcity of research on other crops. Furthermore, the impact of environmental factors on physiological changes in plants remains largely unknown. This review aims to consolidate existing literature on this topic, with a focus on interaction between abiotic stresses (drought, heat, and salinity) and biotic stresses (pathogens and pests). Additionally, it highlights agriculturally important morpho-physiological traits that can be utilized to identify genotypes with combined stress tolerance. Moreover, the review will outline the potential role of recent techniques and genomic tools in unravelling combined stress tolerance in plants. The fndings of this review will help physiologists and molecular biologists to design agronomically relevant strategies for developing broad-spectrum stress-tolerant crops.