Browsing by Author "Singh, Anamika"

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    Chlorpyrifos degradation by Zhihengliuella sp. ISTPL4: An esterase-driven actinobacterial platform for organophosphorus bioremediation
    (Elsevier B.V., 2026) Aggarwal, Himanshi; Chaudhary, Divya; Kumari, Taruna; Pradhan, Nischal; Mishra, Vaibhav; Kumar, Antresh; Singh, Anamika; Pandey, Ashutosh; Chaturvedi, Navaneet; Dufossé, Laurent; Mishra, Arti; Joshi, Naveen Chandra
    Organophosphorus pesticides (OPs) are widely used agrochemicals that pose serious risks to the environmental and human health due to their persistence and toxicity. This study reports, for the first time, chlorpyrifos (CPF) degradation by actinobacterium Zhihengliuella sp. ISTPL4. Strain ISTPL4 utilized various OPs, including dimethoate, monocrotophos, CPF, and malathion, with the highest growth observed in the presence of CPF as the sole carbon and energy source. Optimal growth and degradation occurred at 28 °C, pH 5, and 3% inoculum in minimal salt medium (MSM). Under optimized conditions, strain ISTPL4 degraded 76.95% of 600 mg L-1 CPF within 7 days. GC-MS analysis identified benzene, 1,3-bis(1,1-dimethylethyl) and phenol, 2,4-bis(1,1-dimethylethyl) as intermediates without the formation of toxic metabolite 3,5,6-trichloro-2-pyridinol (TCP). Whole genome analysis revealed five putative esterase genes potentially associated with CPF degradation. Molecular docking identified carboxylesterase B as the most favorable CPF-binding enzyme, while molecular dynamics simulations supported the stability of the enzyme-substrate complex. A putative metabolic pathway for CPF degradation by strain ISTPL4 was proposed. These findings highlight the potential of Zhihengliuella sp. ISTPL4 as a promising candidate for sustainable bioremediation of OP-contaminated environments.
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    MediatorWeb: a protein-protein interaction network database for the RNA polymerase II Mediator complex
    (John Wiley & Sons, 2024) Maji, Sourobh; Waseem, Mohd; Sharma, Manish Kumar; Singh, Maninder; Singh, Anamika; Dwivedi, Nidhi; Thakur, Pallabi; Cooper, David G.; Bisht, Naveen C.; Fassler, Jan S.; Subbarao, Naidu; Khurana, Jitendra P.; Bhavesh, Neel Sarovar; Thakur, Jitendra K.
    The protein-protein interaction (PPI) network of the Mediator complex is very tightly regulated and depends on different developmental and environmental cues. Here, we present an interactive platform for comparative analysis of the Mediator subunits from humans, baker's yeast Saccharomyces cerevisiae, and model plant Arabidopsis thaliana in a user-friendly web-interface database called MediatorWeb. MediatorWeb provides an interface to visualize and analyze the PPI network of Mediator subunits. The database facilitates downloading the untargeted and unweighted network of Mediator complex, its submodules, and individual Mediator subunits to better visualize the importance of individual Mediator subunits or their submodules. Further, MediatorWeb offers network visualization of the Mediator complex and interacting proteins that are functionally annotated. This feature provides clues to understand functions of Mediator subunits in different processes. In an additional tab, MediatorWeb provides quick access to secondary and tertiary structures, as well as residue-level contact information for Mediator subunits in each of the three model organisms. Another useful feature of MediatorWeb is detection of interologs based on orthologous analyses, which can provide clues to understand the functions of Mediator complex in less explored kingdoms. Thus, MediatorWeb and its features can help the user to understand the role of Mediator complex and its subunits in the transcription regulation of gene expression.