Browsing by Author "Raipuria, Ritesh Kumar"

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    Isolation, characterization, and expression analysis of NAC transcription factor from Andrographis paniculata (Burm. f.) nees and their role in andrographolide production
    (MDPI AG, 2024) Kumar, Ramesh; Kumar, Chavlesh; Choudhury, Debjani Roy; Ranjan, Aashish; Raipuria, Ritesh Kumar; Dubey, Kaushik Kumar Dhar; Mishra, Ayushi; Kumar, Chetan; Manzoor, Malik Muzafar; Kumar, Ashok; Kumari, Abha; Singh, Kuldeep; Singh, Gyanendra Pratap; Singh, Rakesh
    Andrographis paniculata (Burm. f.) Nees is an important medicinal plant known for its bioactive compound andrographolide. NAC transcription factors (NAM, ATAF1/2, and CUC2) play a crucial role in secondary metabolite production, stress responses, and plant development through hormonal signaling. In this study, a putative partial transcript of three NAC family genes (ApNAC83, ApNAC21 22 and ApNAC02) was used to isolate full length genes using RACE. Bioinformatics analyses such as protein structure prediction, cis-acting regulatory elements, and gene ontology analysis were performed. Based on in silico predictions, the diterpenoid profiling of the plant’s leaves (five-week-old) and the real-time PCR-based expression analysis of isolated NAC genes under abscisic acid (ABA) treatment were performed. Additionally, the expression analysis of isolated NAC genes under MeJA treatment and transient expression in Nicotiana tabacum was performed. Full-length sequences of three members of the NAC transcription factor family, ApNAC83 (1102 bp), ApNAC21 22 (996 bp), and ApNAC02 (1011 bp), were isolated and subjected to the promoter and gene ontology analysis, which indicated their role in transcriptional regulation, DNA binding, ABA-activated signaling, and stress management. It was observed that ABA treatment leads to a higher accumulation of andrographolide and 14-deoxyandrographolide content, along with the upregulation of ApNAC02 (9.6-fold) and the downregulation of ApNAC83 and ApNAC21 22 in the leaves. With methyl jasmonate treatment, ApNAC21 22 expression decreased, while ApNAC02 increased (1.9-fold), with no significant change being observed in ApNAC83. The transient expression of the isolated NAC genes in a heterologous system (Nicotiana benthamiana) demonstrated their functional transcriptional activity, leading to the upregulation of the NtHMGR gene, which is related to the terpene pathway in tobacco. The expression analysis and heterologous expression of ApNAC21 22 and ApNAC02 indicated their role in andrographolide biosynthesis.
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    Temperature regulation of cell cycle and growth dynamics in Arabidopsis
    (Portland Press, 2026) Sundaravadivelu, Vasundara; Raipuria, Ritesh Kumar; Ranjan, Aashish
    The plant cell cycle is a highly coordinated and regulated process that integrates endogenous and environmental signals to control cell division, meristem maintenance, and cell fate specification for growth and development. Temperature is a critical environmental signal that regulates the cell cycle to manifest developmental plasticity in Arabidopsis roots and shoots. Arabidopsis plants exhibit either adaptive growth responses or arrested growth, depending on the temperature regime. The temperature-mediated growth dynamics in Arabidopsis involve altered cell-cycle regulation. While plant developmental and physiological responses to temperature have been extensively studied, the integration of temperature signalling cues with cell-cycle dynamics to regulate growth adaptation remains poorly understood. The present review not only compiles existing information on temperature-mediated regulation of cell-cycle dynamics but also provides a perspective on multidisciplinary approaches to investigate cell-cycle dynamics at spatiotemporal resolution in Arabidopsis adaptive growth responses.