Browsing by Author "Pawar, Mahendra"

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    Cas variants increased the dimension of the CRISPR tool kit
    (John Wiley & Sons, 2024) Dixit, Sameer; Shukla, Akanchha; Pawar, Mahendra; Vadassery, Jyothilakshmi
    CRISPR technology shows promising developments in multiple areas of biological sciences. It is a developing technology and continuously evolving day by day. Cas proteins, an integral component of CRISPR technology, has enormous scope for new discoveries and reengineering. It can enhance new dimensions in CRISPR tool kit. In this chapter, we systematically explain the classification of CRISPR-Cas system. We separately compiled applicability of different CRISPR-Cas system. In the end, we discussed about the advancement and reengineering of available CRISPR-Cas systems.
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    Plant protease inhibitors: Biological roles and applications in plant stress tolerance
    (Elsevier B.V., 2024) Pawar, Mahendra; Dixit, Sameer; Vadassery, Jyothilakshmi
    Plants have evolved a plethora of defense mechanisms to protect themselves against various biotic and abiotic stresses. Plants are in a constant evolutionary arms race with their attacking insect herbivores and pathogens over a long period of time. Plants when attacked by insects or pathogens use different defense mechanism that involves calcium-signaling, production of reactive oxygen species, phytohormones, miRNAs, secondary metabolites, and defense proteins. The production of protease inhibitors is one such mechanism that contributes to plant defense by severely reducing the growth and development of phytopathogens. Proteases are proteolytic proteins that are necessary for plants to carry out important functions, and the proteolytic activity of proteases needs to be regulated in a controlled manner. Plant protease inhibitors are a family of small protein/peptide/amino acid derivatives that are involved in important biological processes of plants including growth, development, and defense against pests and pathogens (Polya, 2003; Van Der Hoorn, 2008).