Browsing by Author "Patel, Sunita"

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    RAV1 family members function as transcriptional regulators and play a positive role in plant disease resistance
    (John Wiley & Sons, 2023) Chandan, Ravindra Kumar; Kumar, Rahul; Swain, Durga Madhab; Ghosh, Srayan; Bhagat, Prakash Kumar; Patel, Sunita; Bagler, Ganesh; Sinha, Alok Krishna; Jha, Gopaljee
    Phytopathogens pose a severe threat to agriculture and strengthening plant defense response is an important strategy for disease control. Here, we report that AtRAV1, an AP2 and B3 domain-containing transcription factor is required for basal plant defense in Arabidopsis thaliana. The atrav1 mutant lines demonstrate hyper-susceptibility against fungal pathogens (Rhizoctonia solani and Botrytis cinerea) while AtRAV1 overexpressing (OE) lines exhibit disease resistance against them. Enhanced expression of various defense genes and activation of MAP kinases (AtMPK3 and AtMPK6) are observed in the R. solani infected OE lines, but not in the atrav1 mutant plants. In-vitro phosphorylation assay suggests AtRAV1 to be a novel phosphorylation target of AtMPK3. The bimolecular fluorescence complementation and yeast two-hybrid assay support physical interactions between AtRAV1 and AtMPK3. Overexpression of the native as well as phospho-mimic but not the phospho-defective variant of AtRAV1 impart disease resistance in the atrav1 mutant A. thaliana lines. On the other hand, overexpression of AtRAV1 fails to impart disease resistance in the atmpk3 mutant. These analyses emphasize that AtMPK3-mediated phosphorylation of AtRAV1 is important for the elaboration of defense response in A. thaliana. Considering that RAV1 homologs are conserved in diverse plant species, we propose that they can be gainfully deployed to impart disease resistance in agriculturally important crop plants. Indeed, overexpression of SlRAV1 (a member of the RAV1 family) imparts disease tolerance against not only fungal (R. solani and B. cinerea) but also against bacterial (Ralstonia solanacearum) pathogens in tomato, while silencing of the gene enhances disease susceptibility.
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    Silencing of tomato CTR1 provides enhanced tolerance against Tomato leaf curl virus infection
    (Taylor & Francis Group, 2019) Chandan, Ravindra K.; Singh, Achuit K.; Patel, Sunita; Swain, Durga Madhab; Tuteja, Narendra; Jha, Gopaljee
    Tomato leaf curl virus(ToLCV) belonging to Begomovirus family of Geminivirus is known to cause one of the most destructive diseases in tomato. Amongst variousToLCVs, a monopartite Tomato leaf curl Joydebpur virus (ToLCJoV) is most prevalent in eastern part of India. In the present study, we observed induced expression of one of the negative regulators of ethylene signaling pathway gene (LeCTR1)in ToLCJoVinfected plants. TheTobacco rattle virus(TRV) induced silencing of theLeCTR1gene provided enhanced tolerance toToLCJoVinfections. The leaf curling as well as ROS accumulation was significantly reduced in the viral infectedLeCTR1silenced plants. Induction of several defense marker genes (NPR1, PR1, PR5, AOS2, EIN2, EIN3and ERF5) reinforced enhanced tolerance againstToLCJoVinfection in the LeCTR1silenced tomato. Overall, the present study provides evidence that silencing ofLeCTR1can be deployed to protect tomato fromToLCJoVinfections.