Browsing by Author "Pandey, Shubhangi"

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    MPK3 mediated phosphorylation inhibits the dimerization of ABI5 to fine-tune the ABA signaling in Arabidopsis
    (Elsevier B.V., 2025) Bhagat, Prakash Kumar; Verma, Neetu; Pandey, Shubhangi; Verma, Deepanjali; Sinha, Alok Krishna
    Seed germination is, a critical physiological process, is tightly regulated by the phytohormone abscisic acid (ABA). However, the cross talk between multiple regulatory pathways involved in seed germination remains poorly understood. Here, we show that ABA activates two MAP kinases, AtMPK3/AtMPK6, which interact with and phosphorylate AtABI5, a master regulator of ABA signaling. MAP kinase-mediated AtABI5 phosphorylation at the serine-314 position regulates its nuclear localization and dimerization. Interestingly, AtABI5 provides feedback regulation by directly binding to the promoter of AtMPK3 to modulate its transcription. Further, functional analyses revealed that overexpression of a phospho-null AtABI5S314A variant in the abi5-8 mutant background conferred increased ABA sensitivity during seed germination, heightened drought sensitivity, and delayed flowering compared to wild-type plants. Conversely, overexpression of phospho-mimic AtABI5S314D in abi5-8 mutant showed ABA insensitivity during seed germination, drought tolerance, and early floral transition similar to abi5-8 mutant. Collectively, our findings highlight that MAP kinase-mediated phosphorylation of AtABI5 fine-tunes ABA signaling by regulating its dimerization, providing new insights into the dynamic regulation of plant responses to environmental and developmental cues.
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    Transcriptomic and OsWAK24-MPK3 crosstalk reveal resistance mechanism of primed rice seedlings against Arsenic-Iron
    (Oxford University Press, 2026) Bhatia, Priyanka; Mittal, Lavanya; Pandey, Shubhangi; Khatoon, Narjis Saba; Sinha, Alok Krishna; Gupta, Meetu
    Priming, elucidated as "memory," refers to the preconditioning of plant's stress responses to enhance resilience toward future stressors, including arsenic (As) contamination. This adaptive preparedness becomes further complex under As-Fe(iron) interplay, which remains scarcely delineated within priming background. Here, we employed Illumina sequencing to acquire global transcriptome alterations and heterologous interaction approach to chart the molecular reprogramming associated with As and As-Fe-dynamics in primed rice. The transcriptome revealed 3005 and 3650 genes to be differentially regulated in As and As-Fe-exposed primed seedlings. Comprehensive elucidation of expression profiling revealed that key genes were involved in transportation (OsNramp, OsFCR, OsNAS), signalling (OsWRKY, OsMYB, OsAP2, OsZF-TF), and defence (OsHSP, OsPRX, OsCyt P450). The antioxidant (OsGST, OsAPX) expression correlated well with their respective physiological enzyme activity. The entire WAK module, transducing extracellular signals to intercellular pathways, was differentially regulated under As and As-Fe influence. Notably, OsWAK24 revealed higher expression under As-Fe, reflecting its association with seedlings' tolerance behaviour. The computational protein network and docking unfolded OsMPK3 as an interactor of OsWAK24. The split-ubiquitin-based yeast-hybrid assay and immunoblot revealed OsWAK24-OsMPK3 association, suggesting OsMPK3 phosphorylates OsWAK24 at serine residue. Overall, the results decoded the seedling's resilience mechanism and aid in developing rice varieties with desired traits.