Browsing by Author "Mittal, Pramod Kumar"

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    Proteomic profile reveals the diversity and complexity of leaf proteins in spinach (Beta vulgaris var. all green)
    (Proteomics Society, India (PSI), 2016) Ghosh, Sudip; Narula, Kanika; Mittal, Pramod Kumar; Sarkar, Mousumi Poddar; Chakraborty, Niranjan; Chakraborty, Subhra
    Leaf is a source organ that serves dual function in photosynthesis and transpiration. As a primary interface between plant and ecosystem, it performs a range of biological processes from carbon assimilation and metabolite partitioning to plant productivity. Basic features of the leaf functionality are conserved in angiosperms exhibiting common and unique characteristics. Spinach has been the model crop for studying leaf function, primarily photosynthesis. It is a reservoir of several hundreds of primary and secondary biomolecules. To better understand the molecular basis for photochemical reaction and metabolic partitioning, we developed leaf proteome of Indian spinach (Beta vulgaris var. all green). LC-ESI-MS/MS analysis identified 639 proteins exhibiting discrete molecular features and functions, including photosynthesis, transpiration, gaseous exchange, transport, redox status, cell defense, and floral induction besides the presence of proteins with unknown function. This represents the first comprehensive foliage proteome of green leafy vegetable. Together, this work provides important insights into the molecular networks underlying spinach leaf biological processes.