Browsing by Author "Mitra, Souvik"

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    A tripartite interaction among the basidiomycete Rhodotorula mucilaginosa, N2-fixing endobacteria, and rice improves plant nitrogen nutrition
    (American Society of Plant Biologists, 2020) Paul, Karnelia; Saha, Chinmay; Nag, Mayurakshi; Mandal, Drishti; Naiya, Haraprasad; Sen, Diya; Mitra, Souvik; Kumar, Mohit; Bose, Dipayan; Mukherjee, Gairik; Naskar, Nabanita; Lahiri, Susanta; Ghosh, Upal Das; Tripathi, Sudipta; Sarkar, Mousumi Poddar; Banerjee, Manidipa; Kleinert, Aleysia; Valentine, Alexander J.; Tripathy, Sucheta; Sinharoy, Senjuti; Seal, Anindita
    Nitrogen (N) limits crop yield and improvement of N nutrition remains a key goal for crop research; one approach to improve N nutrition is identifying plant-interacting N2-fixing microbes. Rhodotorula mucilaginosa JGTA-S1 is a basidiomycetous yeast endophyte of narrowleaf cattail (Typha angustifolia). JGTA-S1 could not convert nitrate or nitrite to ammonium, but harbors diazotrophic (N2-fixing) endobacteria (eg. Pseudomonas stutzeri) that allows JGTA-S1 to fix N2 and grow in a N-free environment; moreover, P. stutzeri dinitrogen reductase (nifH) was transcribed in JGTA-S1 even under adequate N. Endobacteria-deficient JGTA-S1 had reduced fitness, which was restored by reintroducing P. stutzeri. JGTA-S1 colonizes rice (Oryza sativa) significantly improving its growth, N content, and relative N-use efficiency. Endofungal P. stutzeri plays a significant role in increasing the biomass and ammonium content of rice treated with JGTA-S1; also, JGTA-S1 has better N2 fixing ability than free-living P. stutzeri and provides fixed N to the plant. Genes involved in N metabolism, N transporters, and NODULE INCEPTION (NIN)-like transcription factors were upregulated in rice roots within 24 h of JGTA-S1 treatment. In association with rice, JGTA-S1 has a filamentous phase and P. stutzeri only penetrated filamentous JGTA-S1. Together, these results demonstrate an interkingdom interaction that improves rice N nutrition.